| 透射电镜样品制备技术改良研究 |
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| 引用本文： | 毛晓霞,黄敏毅. 透射电镜样品制备技术改良研究[J]. 现代农业科技, 2013, 0(7): 177+179 |
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| 作者姓名： | 毛晓霞 黄敏毅 |
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| 作者单位： | 安庆师范学院生命科学学院 |
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| 基金项目： | 2012年安庆师范学院青年科研基金项目(自然科学类)(KJ201206);安徽省教育科学规划课题(JG11035) |
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| 摘 要： | 透射电镜样品制备技术改良研究结果表明,通过延长锇酸固定时间和包埋剂梯度渗透时间,避免了细胞器的降解,有利于细胞组织保存和超微结构的观察,并且减少了包埋块切割过程中的破损现象和刻痕,提高了切割质量,此方法能提高生物样品制备质量。
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| 关 键 词： | 透射电子显微镜 样品制备 超微结构 超薄切片 技术改良 |
Study on Improvement of Preparation Technology of TEM Sample |
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| MAO Xiao-xia,HUANG Min-yi. Study on Improvement of Preparation Technology of TEM Sample[J]. Modern Agricultural Sciences and Technology, 2013, 0(7): 177+179 |
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| Authors: | MAO Xiao-xia HUANG Min-yi |
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| Affiliation: | (College of Life Sciences,Anqing Normal University,Anqing Anhui 246011) |
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| Abstract: | The study results of improvement of preparation technolygies of TEM sample showed that extending the time of osmium tetroxide fixed and embedding medium gradient penetration were conducive to degradation of organelle,beneficial to tissue preservation and ultrastructure observation,and could reduce breakage and nicks of embedding block,improve cut quality.This method could improve the quality of the preparation of biological samples. |
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| Keywords: | transmission electron microscope sample preparation ultrastructure ultramicrotome technology improvement |
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