小麦离体叶段鉴定白粉病抗性方法的研究

１９９０年４月至１９９４年４月对小麦离体叶段鉴定白粉病抗性方法进行了研究。结果表明：（１）在灭菌条件下，以在培养皿中垫两层滤纸，用６ＢＡ作保绿主，超过人主至进行鉴定效果较好。（２）保绿剂的浓度低，发病快、退绿早，反之反病慢、退绿晚。其最适浓度是：苗期６ＢＡ为６０－１００ｐｐｍ、苯咪唑为６０ｐｐｍ ；成株期６ＢＡ以８０－１５０ｐｐｍ为佳。（３）份病快、退绿迟的角度出发，鉴定材料以株龄小、叶龄短的初展

STUDIES ON THE DETACHED-LEAF METHOD FOR TESTING RESISTANCE OF WHEAT TO POWERY MILDEW (ERYSIPHE GRAMINIS TRITICI)

The detached-leaf method for testing resistance of wheat to powdery mildew was studied during 1990 - 1994. Results indicated that; (l)Under sterilized conditions, using 2 layers of filter paper in petri dishes containing 6BA(6-benzyl aminopurine)as the greenness preservative and conduction of tests under air-conditional room brought the best results. (2) With lower concentrition of greenness preservatives, the development of powdery mildew was faster, as well as yellowing of the leaf-segments was earlier; whereas the higher concentration could keep the greenness of leaf-segments longer and made the development of symptoms slower. The optimum concentrations for the different preservatives are;on seedling leaves, 60-100ppm of 6BA or 40-60ppm of benzimi-dazole; on adult plant leaves, 80 - 150ppm 6BA. (3) Younger leaves collected from seedlings gave better results than those from adult plants. (4)Results of evaluation for powdery mildew resistance by detached-leaf method were fundamentally the same as those obtained from conventional seedling test method.