| 应用PCR方法检测烟粉虱传双生病毒 |
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| 引用本文： | 叶青静,杨悦俭,王荣青,李志邈,阮美颖,周国治,姚祝平. 应用PCR方法检测烟粉虱传双生病毒[J]. 植物保护, 2008, 34(6): 25-28 |
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| 作者姓名： | 叶青静 杨悦俭 王荣青 李志邈 阮美颖 周国治 姚祝平 |
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| 作者单位： | 浙江省农业科学院蔬菜研究所,杭州,310021 |
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| 基金项目： | 浙江省科技厅资助项目 |
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| 摘 要： | 番茄黄化曲叶病毒属于双生病毒，根据其外壳蛋白基因保守序列设计简并引物，对杭州和海宁地区番茄上疑似番茄黄化曲叶病毒进行PCR检测，获得产物克隆到pMD18-T载体中，经测序和序列分析，表明杭州和海宁地区发生的病毒是番茄黄化曲叶病毒，并且两地区病毒核苷酸序列同源性达97.5%。
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| 关 键 词： | 番茄黄化曲叶病毒 PCR 检测 |
Detection of whitefly-transmitted geminiviruses by reverse transcription-polymerase chain reaction |
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| Ye Qingjing,Yang Yuejian,Wang Rongqing,Li Zhimiao,Ruan Meiying,Zhou Guozhi,Yao Zhuping. Detection of whitefly-transmitted geminiviruses by reverse transcription-polymerase chain reaction[J]. Plant Protection, 2008, 34(6): 25-28 |
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| Authors: | Ye Qingjing Yang Yuejian Wang Rongqing Li Zhimiao Ruan Meiying Zhou Guozhi Yao Zhuping |
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| Affiliation: | Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou310021， China |
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| Abstract: | Tomato yellow leaf curl virus(TYLCV)is a geminivirus transmitted by the whitefly Bemisia tabaci.A pair of degenerate primers was designed according to the conserved sequence of coat protein genes of TYLCV.The tomato samples showing TYLCD-like symptoms were collected from Hangzhou and Haining and detected by PCR for virus presence.PCR products(about 361bp)were then cloned into the vector pMD18-T.Sequence analysis indicated that the nucleotide sequences of the amplified products from Hangzhou and Haining have 97.5% identity,and they share the highest sequence identity with TYLCV,indicating these samples were infected by TYLCV. |
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| Keywords: | Tomato yellow leaf curl virus PCR detection |
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