| 猪线粒体DNA D-loop PCR-RFLP分析 |
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| 引用本文： | 赵兴波,李宁,吴常信.猪线粒体DNA D-loop PCR-RFLP分析[J].中国农业大学学报,1998(5). |
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| 作者姓名： | 赵兴波 李宁 吴常信 |
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| 作者单位： | 中国农业大学（西校区）!北京圆明园西路2号，100094 |
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| 基金项目： | 自然科学基金!“太湖猪高繁殖性能的遗传基础研究” |
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| 摘 要： | 对太湖猪（二花脸）、大约克猪、北京黑猪、五指山猪、香猪、长白猪、杜洛克猪以及太湖猪与7个品种猪的正反交杂交家系线粒体DNAD－loopPCR－RFLP分析，发现猪的线粒体DNAD－loop约为1500bp，长白猪及部分大约克猪与其他猪品种在AccⅠ酶切位点存在多态性；太湖猪、大约克猪的正反交后代的线粒体DNAD－loop在AccⅠ酶切位点存在多态性，其遗传方式遵循母系遗传特性。
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| 关 键 词： | 猪 线粒体DNA D－loop PCR－RFLP 线系遗传 |
PCR-RFLP Analysis of Porcine Mitochondrial DNA D-loop |
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| Zhao Xingbo.PCR-RFLP Analysis of Porcine Mitochondrial DNA D-loop[J].Journal of China Agricultural University,1998(5). |
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| Authors: | Zhao Xingbo |
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| Institution: | Zhao Xingbo (College of Animal Science and Technology) Li Ning (the National Opened Laboratory of Agrobiotechnology)Wu Changxin (College of Animal Science and Technology) |
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| Abstract: | Mitochondrial DNA D-Loops of Taihu (Erhualian),Large White,Beijing Black,Xiang Mini pig, Wuzhishan Mini pig, Landrace,Duroc were amplified and analyzed using 24restriction endonucleases. The molecular size of porcine mitochondrial DNA D-loop amplified was about 1 500 bp, and the cleavage patterns of 24 restriction endonucleases, except Acc1 were identical among all pigs examined, the D-loop digested by Accl was polymorphic that had 150bp and 1 350 bp fragments in all Landrace and parts of Large White while other breeds' D-loop had no cleavage site. the restriction patterns of reciprocal cross of family line between Taihu and Large White showed maternal inheritance. |
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| Keywords: | pig mitochondrial DNA D-loop PCR-RFLP maternal inheritance |
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