Cloning, Sequencing and Expression of a Xylanase Gene from the Maize Pathogen Helminthosporium Turcicum |
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Authors: | Yeshitila Degefu Lars Paulin Peter Stephensen Lübeck |
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Affiliation: | (1) Department of Applied Biology, Plant and Forest Pathology, University of Helsinki, P.O. Box 27, Helsinki, FIN-00014, Finland;(2) Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki, FIN-00014, Finland;(3) Department of Microbiology, Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Copenhagen, Denmark |
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Abstract: | A gene encoding an endoxylanase from the phytopathogenic fungus Helminthosporium turcicum Pass. was cloned and sequenced. The entire nucleotide sequence of a 1991 bp genomic fragment containing an endoxylanase gene was determined. The xylanase gene of 795 bp, interrupted by two introns of 52 and 62 bp, encoded a protein of 227 amino acids showing up to 95% amino acid homology with other fungal xylanases. The precise splicing site of the introns was identified by sequencing the corresponding cDNA. A northern blot showed that the gene is expressed when the fungus is grown in a medium containing xylan as a sole carbon source. The cloned xylanase gene was expressed in maize plants during infection. |
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Keywords: | Endoxylanase Helminthosporium turcicum Zea mays |
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