瓦克青霉F10-2对川楝树皮残渣纤维素酶解研究

以川楝树皮残渣为原料进行纤维素酶解研究,测定了不同培养时间培养基质中主要胞外酶活性的变化,并对发酵前后残渣结构进行扫描电镜观察和红外光谱分析。结果表明:瓦克青霉F10-2具有木质纤维素降解能力,酶解液中纤维素酶、半纤维素酶、木质素过氧化物酶和锰过氧化物酶活性在发酵的8～16d内分别达到最大值6.42U·g-1、7.62U·g-1、6.55U·g-1和3.33U·g-1。利用扫描电镜对降解后底物表面结构进行观察,可看到残渣表面变得疏松、柔软,且具有部分微孔。底物残渣傅立叶红外光谱分析表明,该菌株对残渣中各组分均有一定降解。固态发酵培养试验表明,培养24d后残渣中纤维素、半纤维素和木质素的降解率分别达到42.70%、33.96%和24.62%。

Enzymatic hydrolysis of chinaberry bark residue by Penicillium waksmanii F10-2

The extra-cellular enzyme activity of Penicillium waksmanii F10-2 was determined using chinaberry bark residues as substrate at different times. The ultra-structure and chemical constituents of the residues before and after fermentation were also compared by using scanning electron microscope (SEM) and Fourier transform infrared spectrum (FTIR). The results show that fermentation of P. waksmanii F10-2 utilizes methyl cellulose as substrate. After 8~16 days of fermentation, the maximum activities of cellulase, hemi-cellulase, lignin peroxidase and Mn-depended peroxidase are 6.42 U·g^-1, 7.62 U·g^-1, 6.55 U·g^-1 and 3.33 U·g^-1, respectively. The ultra-structure of degraded chinaberry bark residues is soft and loose with some micro-pores. FTIR spectrum analysis of the residues shows that P. waksmanii fermentation products degrade components of chinaberry bark residues. After fermentation for 24 d, the ratios of degradation of cellulose, hemi-cellulose and lignin in residues are 42.70%, 33.96% and 24.62%, respectively.