Immunoblot analysis of specific antigen bands predictable for Dirofilaria immitis infection in cats |
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| Authors: | Hayasaki Mineo Mori Naoko Hongo Haruka Katsuya Akiyo Song Kun-Ho Une Satoshi Ikeda Tadao Ooi Hong-Kean Uchida Akihiko |
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| Affiliation: | Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. hayasaki@yamaguchi-u.ac.jp |
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| Abstract: | Serial sera from four mongrel cats experimentally inoculated with infectious larvae of Dirofilaria immitis were analyzed by immunoblot patterns against a phosphate buffered saline-extract of D. immitis. Antigen-specific protein bands detected indicate that the low molecular weight bands of 36, 32, 22, 19 and 14 kDa, are predictable for positive adult worm infection, suggesting diagnostic usefulness for adult D. immitis infection in cats. |
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