SSR-tagged breeding scheme for allogamous crops: a trial in bunching onion (<Emphasis Type="Italic">Allium fistulosum</Emphasis>) |
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Authors: | Hikaru Tsukazaki Ken-Ichiro Yamashita Akio Kojima Tadayuki Wako |
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Institution: | (1) National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu Mie, 514-2392, Japan |
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Abstract: | In several autogamous and vegetatively propagated crops, DNA markers have been used for cultivar identification. However,
allogamous crops such as bunching onion (Allium fistulosum L.) are recalcitrant to marker-aided cultivar identification, as well as hybrid seed purity tests, due to the high degree
of genetic heterogeneity within each cultivar. To aid cultivar identification and ensure its accuracy in bunching onion, we
proposed the “SSR-tagged breeding” scheme in our previous study. The feasibility of this scheme was investigated here using
a landrace of bunching onion with two populations tagged with two or four selected SSR markers. Compared with a control population,
no significant differences were detected in the agronomic traits of the SSR-tagged populations. The targeted SSR loci were
genetically uniform within each population whereas other loci maintained high heterogeneity. These results demonstrate that
the SSR-tagged breeding scheme, even with a very small number of markers, is efficient for the identification of newly bred
cultivars, and consequently for F1 purity tests, in allogamous crops in which inbreeding depression is as severe as in bunching onion. |
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Keywords: | Allium fistulosum Allogamous crop Bunching onion Genetic heterogeneity SSR-tagged breeding |
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