贮藏方式和贮藏时间对苜蓿干草霉菌数量和种类的影响

为探讨贮藏方式和贮藏时间对苜蓿(Medicago sativa)干草霉菌数量和种类的影响,本研究在甘肃省酒泉市肃州区采集露天贮藏、苫布贮藏和储草棚贮藏下不同贮藏时间的苜蓿干草,用PD A平皿培养法、形态鉴定法和rD-NA-ITS序列分析法对霉菌的数量和种类进行了鉴定和分析.结果表明:贮藏方式和时间对苜蓿干草的霉菌数量和...

Effects of Storage Methods and Time on Mould Quantity and Species in Alfalfa Hay

In order to explore the effects of storage methods and time on mould quantity and species in alfalfa hay,different stored samples were taken from hay harvested in Suzhou District of Jiuquan City in Gnasu Province,including stored outdoors with or without tarp cover and under shelter with 360 days storage period. PDA plate culture,morphological identification and rDNA-ITS sequence were used to detect and identify mould quantity and species. The results showed that both storage methods and time had significant influences on fungi quantity and species. During 360 days storage period,the mould quantity increased generally. Among 3 storage methods,alfalfa hay stored outdoors without tarp cover had the greatest moulds counts and fluctuation (4.57×10³~1.74×10⁴ count·g^-1),followed by hay stored outdoors with tarp cover. The least moulds counts and variation (3.31×10³~7.29×10³count·g^-1) were observed in alfalfa hay stored under shelter. A total of 41 fungi species of 16 genera were detected,and the dominant genera were Alternaria sp.,Cladosporium sp. and Aspergillus sp..With increasing storage time,the number of detected fungi species showed an increasing-reducing-increasing trend. Alfalfa hay stored under shelter showed the least variation. In conclusion,alfalfa hay in Hexi Corridor of Gansu Province should be stored under shelter with no longer than 7 months of storage time.