狼尾草属象草PpPAPs基因的克隆及响应低磷胁迫的表达模式分析

为解析象草(Pennisetum purpureum)适应低磷胁迫机理,本研究通过水培试验,分析了不同磷源处理对象草生长的影响,并利用同源克隆和cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)技术,从象草中克隆了3个可能参与ATP利用的PA P基因.结果表明,在腺嘌呤核...

Molecular Cloning of PpPAP Genes and Their Expression Responses to Phosphorus Deficiency in Elephant Grass (Pennisetum purpureum)

To insight into mechanisms of elephant grass adapting to low phosphorus(P)stress,the effects of different P sources on elephant grass growth were investigated by hydroponic experiments.Three PpPAP genes related to ATP utilization were cloned from elephant grass by homologous cloning and rapid amplification of cDNA ends(RACE)technology.Results showed that dry weight and total P content at the application of adenosine triphosphate(+ATP)treatment were significantly higher than those at low P(-P)treatment,suggesting that elephant grass has a strong ability to utilize ATP.QRT-PCR analysis showed that after 10 days of growth,the expression of these three PpPAPs associated with ATP utilization in the roots was 170%~403%greater than that of in+ATP treated plants compared to those grown under normal P(+P)treatment.Furthermore,+ATP treatment led to significant increases in the activities of root intracellular and secreted acid phosphatase(ACP),compared to+P treatment.Taken together,the results herein suggest that the expression of PpPAP genes in roots of elephant grass were up-regulated by P deficiency,which may promote the synthesis and secretion of ACP.These adaptive responses of elephant grass may contribute to the utilization of ATP under low P stress.