| 重组MBP-GnRH6融合蛋白的表达与鉴定 |
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| 引用本文： | 王索路,方富贵,刘亚,章孝荣,李运生,张运海,陶勇,曹鸿国. 重组MBP-GnRH6融合蛋白的表达与鉴定[J]. 农业生物技术学报, 2010, 18(2): 260-264. DOI: 10.3969/j.issn.1674-7968.2010.02.009 |
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| 作者姓名： | 王索路 方富贵 刘亚 章孝荣 李运生 张运海 陶勇 曹鸿国 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥,230036 |
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| 基金项目： | 安徽省教育厅自然科研项目 |
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| 摘 要： | 将人工合成促性腺激素释放激素(GnRH)六聚体基因片段连接至pMAL-c4x载体,获得的pMAL-c4x-GnRH6重组质粒转化到大肠杆菌TB1中进行表达.采用SDS-PAGE,Western blot进行鉴定,并通过动物实验对表达产物的免疫原性进行检测.获得了分子量大小约为50 kD的麦芽糖结合蛋白-GnRH六聚体(MBP-GnRH6),且融合蛋白与GnRH抗体的反应性较好,并能使睾丸萎缩、变性.表明重组MBP-GnRH6融合蛋白具有较好的反应原性和免疫原性,可用于小鼠的免疫去势.
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| 关 键 词： | GnRH六聚体 pMAL-c4x质粒 MBP-GnRH6融合蛋白 诱导表达 免疫原性 |
Expression and Identification of Recombination MBP-GnRH6 Fusion Protein |
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| Wang Suolu,Fang Fugui,Liu Ya,Zhang Xiaorong,Li Yunsheng,Zhang Yunhai,Tao Yong,Cao Hongguo. Expression and Identification of Recombination MBP-GnRH6 Fusion Protein[J]. Journal of Agricultural Biotechnology, 2010, 18(2): 260-264. DOI: 10.3969/j.issn.1674-7968.2010.02.009 |
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| Authors: | Wang Suolu Fang Fugui Liu Ya Zhang Xiaorong Li Yunsheng Zhang Yunhai Tao Yong Cao Hongguo |
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| Affiliation: | WangSuolu FangFugui LiuYa ZhangXiaorong LiYunsheng ZhangYunhai TaoYong CaoHongguo Animal Technological College,Anhui Agricultural University,Hefei,230036 |
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| Abstract: | The gene fragment of the gonadotropin-releasing hormone(GnRH) hexamer was connected to the pMALc4x plasmid, then recombinant pMAL-c4x-GnRH6 was induced in Escherichia coli TB1 by IPTG.SDS-PAGE, Western blot and animal experimentation was used to identify the expression product.The molecular weight of fusion protein MBP-GnRH6 was about 50 kD.And the reactivity of fusion protein and GnRH-antibody was better.Active immunization against maltose-bindingprotein and gonadotropin-releasinghormone hexamer(MBP-GnRH6)... |
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| Keywords: | GnRH hexamer pMAL-c4x Fusion protein of MBP-GnRH6 Induction and expression Immunogenicity |
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