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| 无核雪柑与普通雪柑种胚发育过程蛋白质的差异 | |
| 引用本文： | 王玉玲,陈清西,陈伟. 无核雪柑与普通雪柑种胚发育过程蛋白质的差异[J]. 福建农业学报, 2007, 22(2): 158-161 |
| 作者姓名： | 王玉玲 陈清西 陈伟 |
| 作者单位： | 1. 漳州师范学院生物科学与技术系,福建,漳州,363000;福建农林大学园艺学院,福建,福州,350002 2. 福建农林大学园艺学院,福建,福州,350002 3. 福建农林大学生命科学学院植物蛋白质组学实验室,福建,福州,350002 |
| 摘 要： | 采用考马斯亮蓝G 250法测定胚胎蛋白质含量,并用双向电泳技术分析胚胎的特异蛋白质。结果表明:无核雪柑种胚早期的蛋白质含量相对较高,其胚胎不同发育阶段出现了P1~P6 6个特异蛋白质;另外发现了4个特异于普通雪柑胚胎发育的蛋白质P11~P14。认为P11~P14与无核雪柑的无核密切相关,分别是P11(67.0 kD,pI 5.1)、P12(65.5 kD,pI 5.4)、P13(97.0 kD,pI 5.8)及P14(64.3 kD,pI 5.9)。 |
| 关 键 词： | 无核雪柑 胚胎发育 特异蛋白质 |
| 文章编号： | 1008-0384（2007）02-0158-04 |
| 收稿时间： | 2007-02-10 |
| 修稿时间： | 2007-05-22 |
Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage |
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| WANG Yu-ling,CHEN Qing-xi,CHEN Wei. Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 158-161 | |
| Authors: | WANG Yu-ling CHEN Qing-xi CHEN Wei |
| Affiliation: | 1. Department of Biological Science and Biotechnology , Zhangzhou Normal University, Zhangzhou , Fujian 363000, China; 2. Horticultural College, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China; 3. Plant Proteomics Laboratory of College of Life Science Fujian Agricultural and Forestry University ~ Fuzhou~ Fujlan 350002~ China |
| Abstract: | The method of Coomassie brilliant blue was used to determine the protein content in embryos.It indicated that the protein content in "Wuhexuegan" was higher than that in Citrus sinesis Osbeck at the early stage of embryo development.In order to study the specific proteins of "Wuhexuegan" at the embryonic development stage,two-dimensional electrophoresis technique was employed.As a result,6 new proteins at different stages of "Wuhexuegan" embryo development and 4 specific proteins of "Wuhexuegan" that were different from Citrus sinesis Osbeck in the embryonic development stage were identified.The 4 proteins were P11(67.0kD,pI 5.1),P12(65.5kD,pI 5.4,P13(97.0kD,pI 5.8),and P14(64.3kD,pI 5.9).Therefore,it is suggested that these 4 specific proteins might play an important role in "Wuhexuegan" embryo differentiation and development. |
| Keywords: | Wuhexuegan Citrus sinesis Osbeck embryo development specific protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |