“畜禽用多种微量元素注射液”对小鼠免疫功能的影响

将60只昆明小鼠(体质量为18~22 g,雌雄各半)随机分为3组,每组20只。再将每组的20只分成试验组和对照组,每组10只,试验组每天腹腔注射0.04 mL"多种微量元素注射液",对照组注射0.04 mL生理盐水,连续注射7 d。3组分别进行酸性α-醋酸萘脂酶法试验,溶血空斑试验和血清溶血素抗体(HC50)测定。结果表明,试验组小鼠ANAE阳性细胞百分比为51.80%±3.645%,极显著高于对照组的39.75%±4.283%(P0.01);试验组小鼠溶血空斑形成数量为23.1±2.13177,极显著高于对照组的17.4±2.01108(P0.01);试验组小鼠血清溶血素水平为262.2900±10.48257,显著高于对照组的231.2710±39.61145(P0.05)。即"畜禽用多种微量元素注射液"能显著提高小鼠的免疫功能。

Effect of Injection of Trace Elements for Livestock and Poultry on the Immune Function of the Mice

60 Mice were randomly divided into three groups, and there are 20 mice in every group, meanwhile 20 mice were averagely divided into control group and test group. Mice in test group were injected intraperitoneally with 0.04 mL "Trace elements injection", and mice in control group with the same volume saline water once a day, continuously seven days. The ANAE test, hemolytic plaque test and content of serum hemolysin HC_(50) were carried out. The percentage of positive ANAE cell(51.80%±3.645%) in test group is extremely obviously higher than that of control group(39.75%±4.283%)(P< 0.01). Hemolytic plaque quantity(23.1±2.13177) in test group is extremely obviously higher than that of control group(17.4±2.01108)(P< 0.01).Serum hemolysin (262.2900±10.48257) in test group is obviously higher than that of control group(231.2710±39.61145)(P< 0.05). These results suggested that "Trace elements injection" can obviously elevate immunologic funtion of mice.