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双翅目昆虫对荷斯坦奶牛护身行为及外周血液中淋巴细胞亚群的影响
引用本文:吴德胜,王纯洁,敖日格乐.双翅目昆虫对荷斯坦奶牛护身行为及外周血液中淋巴细胞亚群的影响[J].黑龙江畜牧兽医,2009(7).
作者姓名:吴德胜  王纯洁  敖日格乐
作者单位:内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018 
摘    要:为了研究荷斯坦奶牛受到双翅目昆虫侵袭时奶牛个体所表现的护身行为、外周血液中淋巴细胞亚群的变化规律,试验期间每日跟踪观察奶牛护身行为1 h,记录每5 min内侵袭奶牛的双翅目昆虫数目和奶牛的护身行为出现次数,定时采血用流式细胞仪检测外周血液中CD3+、CD4+、CD8+ T淋巴细胞和CD21+B淋巴细胞等的变化规律.结果表明:奶牛护身行为随飞来昆虫的数量而变化;外周血液中的CD3+、CD4+、CD21+水平随双翅目昆虫数量的增多而降低;CD8+水平随双翅目昆虫数量的增多而升高.

关 键 词:荷斯坦奶牛  双翅目昆虫  护身行为  淋巴细胞亚群

The effect of diptera insect on shielding action and lymphocytes subsets in peripheral blood of Holstein dairy cows
WU De-sheng,WANG Chun-jie,AORIGELE.The effect of diptera insect on shielding action and lymphocytes subsets in peripheral blood of Holstein dairy cows[J].Heilongjiang Animal Science And veterinary Medicine,2009(7).
Authors:WU De-sheng  WANG Chun-jie  AORIGELE
Institution:College of Animal Science and Veterinary Medicine;Inner Mongolia Agricultural University;Huhhot 010018;China
Abstract:In order to study the changes of shielding action,the peripheral blood lymphocytes and their subsets of Holstein dairy cows in stress of diptera invasion,the numbers of shielding action and diptera insect invasion were surveyed and recorded each interval 5 min in 1 h daily.Blood were taken regularly.CD3+,CD4+,CD8+ T cells and CD21+ B cells changes of peripheral blood were detected by flow cytometry.The results show that shielding action of Holstein dairy cows changed with the number of diptera insect,CD3+,C...
Keywords:Holstein dairy cows  diptera insect  shielding action  lymphocytes subsets  
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