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复合菌系XDC-2分解未经化学处理的水稻秸秆
引用本文:王 慧,刘小平,郭 鹏,高瑞芳,王小芬,崔宗均.复合菌系XDC-2分解未经化学处理的水稻秸秆[J].农业工程学报,2011,27(13):86-90.
作者姓名:王 慧  刘小平  郭 鹏  高瑞芳  王小芬  崔宗均
作者单位:1. 中国农业大学农学与生物技术学院/生物质工程中心,北京 100193;4. 山东省金乡县农业局植保站,济宁 272200;2. 湖北农业科学院农产品加工与核农技术研究所,武汉 430064; 3. 国家食用菌加工技术研发分中心,武汉 430064;1. 中国农业大学农学与生物技术学院/生物质工程中心,北京 100193;1. 中国农业大学农学与生物技术学院/生物质工程中心,北京 100193;1. 中国农业大学农学与生物技术学院/生物质工程中心,北京 100193
基金项目:国家“十一五”支撑计划重大项目(No. 2006BAD07A01, No. 2006BAD25B04)
摘    要:利用最近筛选得到的一组木质纤维素分解复合菌系XDC-2分解未经任何化学方法预处理的水稻秸秆,结果表明,接种后第6 d,胞外木聚糖酶活达335 U/mL。经分解12 d,水稻秸秆总质量减少率达39.71%,其中半纤维素质量减少率为78.27%,纤维素质量减少率为14.08%。在分解第6 d,产物种类及含量均达到最多,共检测出4主要的物质分别为乙酸、丙酸、丁酸和甘油,这些有机物均可在工业生产中得到转化和利用。DGGE分析表明,复合菌系XDC-2在分解未处理水稻秸秆过程中的微生物组成结构稳定。结果表明,复合菌系XCD-2对于未经任何化学方法预处理的水稻秸秆分解能力强,极具开发利用潜力。

关 键 词:农业废弃物,发酵,生物质,未处理水稻秸秆,复合系XDC-2,胞外木聚糖酶活
收稿时间:2011/1/10 0:00:00
修稿时间:2011/3/16 0:00:00

Degradation of rice straw without chemical pretreatment by a composite microbial system XDC-2
Wang Hui,Liu Xiaoping,Guo Peng,Gao Ruifang,Wang Xiaofen and Cui Zongjun.Degradation of rice straw without chemical pretreatment by a composite microbial system XDC-2[J].Transactions of the Chinese Society of Agricultural Engineering,2011,27(13):86-90.
Authors:Wang Hui  Liu Xiaoping  Guo Peng  Gao Ruifang  Wang Xiaofen and Cui Zongjun
Abstract:The degradation of rice straw without any chemical method pretreated by a lignocellulose degradation composite microbial system XDC-2 was studied. After 6 days cultivation, exocellular xylanase activity was 335 U/mL. After 12 days, the rice straw lost 39.71% of its weight, and the weight loss of hemicellulose and cellulose were 78.27% and 14.08% respectively. The total amount of the volatile products came to a maximum in the sixth day. Four major kinds of the volatile products were determined in fermentation broth, they were acetic acid, propionic acid, butanoic acid and glycerin, which could be utilized in industrial production. The composite microbial system XDC-2 became stable in degradation process of rice straw without any chemical method pretreated according to PCR-DGGE analysis. Accordingly, it has the potential for further development and application of composite microbial system XDC-2 due to its effective ability to degrade rice straw without any chemical pretreatment.
Keywords:agricultural wastes  fermentation  biomass  un-pretreated rice straw  composite microbial system XDC-2  exocellular xylanase
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