水稻叶绿体的petG-trnP片段的测定和系统学分析*

本研究主要对水稻的叶绿体petG-trnP片段进行PCR直接测序和系统学分析。结果表明，水稻的petG-trnP的序列长度十分保守，其间隔区全长为316bp，扣除trnW序列后的非编码区长为242bp，其中两段非编码区分别为116bp和126bp。非编码区中G+C含量在37.19%-38.02%之间。对五种水稻材料进行ClustalW等分析表明，它们的序列结构有所变异，共有7个变异位点，比例为2.89%，这预示着水稻的petG-trnP的非编码区中有着一定的系统学信息，可在分子水平上为稻属的系统关系提供一定的证据。

Study on the sequencing and phylogenetics analysis of the rice chloroplast DNA petG-trnP fragment

The rice chloroplast petG-trnP fragment was sequenced and analyzed in this study.The results showed that the petG-trnP intergenic spacer was very conservative,and its length was 316 bp.The length of non-coding region was 242 bp deducting the trnW sequence,and the two ends of the non-coding region were 116 bp and 126 bp,respectively.The G＋C content ranged from 37.19% to 38.02% in the non-coding region.The ClustalW analysis showed that the petG-trnP fragment structure was variable,and there were 7 variable sites （2.89%） in the non-coding region.The results indicated that the non-coding region of petG-trnP contained some phylogenetic information,and could provide the direct molecular evidence to clarify the Oryza species origin and evolution.