| 大鼠热休克因子结合蛋白1原核表达载体的构建及表达 |
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| 引用本文： | 马辉,赵绪永. 大鼠热休克因子结合蛋白1原核表达载体的构建及表达[J]. 郑州牧业工程高等专科学校学报, 2007, 27(4): 4-6 |
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| 作者姓名： | 马辉 赵绪永 |
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| 作者单位： | 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011 |
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| 摘 要： | 以重组克隆载体pGEM—T—hsbp1为模板,PCR扩增hsbp1,用限制性内切酶双酶切、T4连接酶连接、转化BL -21的方法构建pGEX-2TK-hsbp1原核表达载体,实现了hsbp1在BL21中的表达,SDS—PAGE检测表达的融合蛋白主要以可溶形式存在,并利用亲和层析得到融合蛋白。
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| 关 键 词： | 原核表达 亲和层析 |
| 文章编号： | 1008-3111（2007）04-0004-03 |
| 收稿时间： | 2007-02-12 |
| 修稿时间： | 2007-02-12 |
Construction of pGEX-2TK-Hsbp1 Prokaryotic Expression Vector and Expression of HSBP1 |
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| MA Hui,ZHAO Xu-yong. Construction of pGEX-2TK-Hsbp1 Prokaryotic Expression Vector and Expression of HSBP1[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 2007, 27(4): 4-6 |
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| Authors: | MA Hui ZHAO Xu-yong |
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| Abstract: | Using recombination cloning plasmid pGEM-T-hsbp1 as template,heat shock factor binding protein gene was cloned;By restriction endonuclease,T4 DNA ligase,BL-21,the pGEX-2TK-hsbpl prokaryotic expression vector was constructed.The recombinant expression vector can express fusion proteins of recombinant HSBP1 in BL21.The result of SDS-PAGE manifests that the target protein was expressed in soluble form.We obtained target protein by affinity chromatography. |
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| Keywords: | hsbp1 SDS-PAGE |
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