| 用RT-PCR技术检测盐渍猪肠衣中猪瘟病毒的研究 |
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| 引用本文： | 朱中武,唐连飞,鲁杏华,姜金林,孟芳,朱金国,李丹丹,向俨. 用RT-PCR技术检测盐渍猪肠衣中猪瘟病毒的研究[J]. 中国预防兽医学报, 2006, 28(6): 681-683 |
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| 作者姓名： | 朱中武 唐连飞 鲁杏华 姜金林 孟芳 朱金国 李丹丹 向俨 |
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| 作者单位： | 1. 湖南出入境检验检疫局检验检疫技术中心,湖南,长沙,410007
2. 湖南省畜牧兽医总站,湖南,长沙,410007 |
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| 摘 要： | 参考GenBank中发表的猪瘟病毒（CSFV）序列，设计一对CSFV特异性PCR引物；从CSFV感染猪盐渍小肠中提取总RNA，经逆转录后进行PCR扩增，在盐渍小肠中成功扩增出与预期大小（168bp）一致的特异性条带，而正常猪和感染猪伪狂犬病病毒的猪小肠扩增结果均为阴性。用本方法对20例不同稀释浓度的盐渍猪肠衣样本进行检测，结果显示比经典抗原检测方法（抗原捕获ELISA法）具有更高的敏感性。实验表明，本RT—PCR技术能应用于盐渍猪肠衣的CSFV检测，为快速、准确检测盐渍猪肠衣中CSFV提供了一条新途径。
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| 关 键 词： | 猪瘟病毒 盐渍猪肠衣 |
| 文章编号： | 1008-0589(2006)06-0681-03 |
| 收稿时间： | 2005-08-25 |
| 修稿时间： | 2005-08-25 |
Detection of classical swine fever virus in salted swine casings by RT-PCR |
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| ZHU Zhong-wu,TANG Lian-fei,LU Xing-hua,JIANG Jin-lin,MENG Fang,ZHU Jin-guo,LI Dan-dan,XIANG Yan. Detection of classical swine fever virus in salted swine casings by RT-PCR[J]. Chinese Journal of Preventive Veterinary Medicine, 2006, 28(6): 681-683 |
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| Authors: | ZHU Zhong-wu TANG Lian-fei LU Xing-hua JIANG Jin-lin MENG Fang ZHU Jin-guo LI Dan-dan XIANG Yan |
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| Affiliation: | 1. Inspection and Quarantine Technology Centre, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410007, China ; 2. General Animal Husbandry and Veterinary Station of Hunan Province, Changsha 410007, China |
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| Abstract: | A pair of specific primers was synthesized according to the sequences of classical swine fever virus(CSFV)pub- lished in GenBank.The specific expected fragment(168 bp)was amplified by RT-PCR from the total RNA extracted from salted casings of pigs infected CSFV whereas no amplification were detected in normal or pseudorabies virus(PRV)infected.Twenty salted swine casing samples were detected by this method,which showed the same results as antigen capture ELISA test.This method can be used for the fast and accurate detection of CSFV in salted swine casings. |
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| Keywords: | RT-PCR |
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