| 克隆全长cDNA的方法及其在兽药研究中的应用 |
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| 引用本文： | 谢馥交,卢向阳. 克隆全长cDNA的方法及其在兽药研究中的应用[J]. 中国兽药杂志, 2006, 40(5): 39-43 |
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| 作者姓名： | 谢馥交 卢向阳 |
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| 作者单位： | 湖南农业大学生物科学与技术学院,湖南,长沙,410128 |
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| 摘 要： | 综述了获取基因全长cDNA序列的方法,即全长cDNA文库的构建、cDNA末端快速扩增和电子克隆;重点介绍了Oligo-capping、CAPture、Cap-trapper及SMART构建全长cDNA文库的方法,并阐述了其在兽药研究中的应用.
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| 关 键 词： | 全长cDNA 全长cDNA文库 cDNA末端快速扩增 电子克隆 |
| 文章编号： | 1002-1280（2006）05-0039-05 |
| 收稿时间： | 2005-03-15 |
| 修稿时间： | 2005-03-15 |
Methods for Full-length cDNA Cloning and Their Application in Veterinary Drug Research |
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| XIE Fu-jiao,LU Xiang-yang. Methods for Full-length cDNA Cloning and Their Application in Veterinary Drug Research[J]. Chinese Journal of Veterinary Drug, 2006, 40(5): 39-43 |
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| Authors: | XIE Fu-jiao LU Xiang-yang |
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| Affiliation: | Department of Biologic Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128; China |
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| Abstract: | The cloning of full-length cDNA is the prerequisite for the gene structure and function analysis.(Methods) for full-length cDNA cloning,such as full-length cDNA library(Oligo-capping,CAPture,Cap-trapper,Cap-jumping and SMART),RACE,in-silico cloning are displayed.The applications of these methods in veterinary drug research are also mentioned in this paper. |
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| Keywords: | full-length cDNA cDNA library RACE in-silico cloning |
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