Nanoscopy in a living mouse brain |
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Authors: | Berning Sebastian Willig Katrin I Steffens Heinz Dibaj Payam Hell Stefan W |
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Affiliation: | Department of NanoBiophotonics, Max Planck Institute (MPI) for Biophysical Chemistry, G?ttingen, Germany. |
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Abstract: | We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time. |
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