蕹菜梗叶绿素浸提工艺的优化及其理化性质

利用单因子试验和正交试验探讨了蕹菜梗叶绿素的最佳浸提工艺，并对其理化性质进行分析。结果表明，蕹菜梗叶绿索的最佳浸提工艺为：乙醇浓度85％、料液比1：50、提取温度70℃、提取时间4h，其提取率为0．1957mg·g^-1；蕹菜梗叶绿素易溶于有机溶剂，但不溶于水，其可见光区的最大吸收波长为410nm，且具有较好的耐光和耐热性。

Optimization of extraction of chlorophyll from water spinach stems by orthogonal design and physicochemical properties

In this paper, the stem of water spinach was used as test material for extraction of chlorophyll. The single factor test and orthogonal design was adopted to study the optimal conditions for the extraction of chlorophyll from water spinach stems. Moreover, its physicochemical properties were further studied. The results showed that the optimal conditions were obtained as follows： 85% ethanol, ratio of material to extractant 1 ： 50, extraction temperature 70 ℃ and extraction time 4.0 hours. Under the optimal condi- tions, the extraction ratio of chlorophyll was 0.1957 mg · g- 1. The chlorophyll was soluble in ethanol, methanol, acetic ether and diethyl, but insoluble in water. The maximal absorbing-wavelength was 410 nm. The study also indicated that chlorophyll bad a good tolerance for thermo and photo-light.