| 高效液相色谱法测定乳粉中5种核苷酸的含量 |
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| 引用本文： | 赵贞,岳虹,刘春霞,李素琴,陈静,李翠枝. 高效液相色谱法测定乳粉中5种核苷酸的含量[J]. 乳业科学与技术, 2015, 38(3): 26-29. DOI: 10.15922/j.cnki.jdst.2015.03.007 |
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| 作者姓名： | 赵贞 岳虹 刘春霞 李素琴 陈静 李翠枝 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特,010110 |
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| 基金项目： | “十二五”国家科技部支撑计划项目(2012BAK17B00) |
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| 摘 要： | 建立乳粉中核苷酸高效液相色谱的测定方法.采用超纯水作为提取剂,0.5％的乙酸沉淀蛋白,SAX固相萃取小柱净化,C18色谱柱分离,流动相为0.1 mol/L的磷酸二氢钾溶液.每100 g乳粉中核苷酸添加量为0.3～50 mg时,回收率大于90％,定量限为0.3 mg/100 g.该方法具有结果准确、操作简便、重复性好等优点,可用于乳粉中核苷酸的检测.
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| 关 键 词： | 高效液相色谱法；核苷酸；SAX固相萃取小柱；乳粉 |
High Performance Liquid Chromatography Determination of 5 Nucleotides in Milk Powder |
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| ZHAO Zhen,YUE Hong,LIU Chunxia,LI Suqin,CHEN Jing,LI Cuizhi. High Performance Liquid Chromatography Determination of 5 Nucleotides in Milk Powder[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2015, 38(3): 26-29. DOI: 10.15922/j.cnki.jdst.2015.03.007 |
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| Authors: | ZHAO Zhen YUE Hong LIU Chunxia LI Suqin CHEN Jing LI Cuizhi |
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| Affiliation: | (Inner Mongolia Yili Industrial Group Co.Ltd., Hohhot 010110, China) |
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| Abstract: | A method for the determination of nucleotides in milk powder by high performance liquid chromatography (HPLC)was developed. Nucleotides were extracted with water, deproteinated with 0.5% acetic acid and cleaned up by an SAXsolid phase extraction (SPE) column. The analysis was performed on C18 column using 0.1 mol/L phosphate as the mobilephase. The recoveries of spiked samples at three levels ranging from 0.3 to 50 mg/100 g were higher than 90%. The limit ofquantification was 0.3 mg/100 g. This method was accurate, simple, reproducible, and suitable for the analysis of nucleotidesin milk powder. |
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| Keywords: | high performance liquid chromatography (HPLC) nucleotides SAX SPE column milk powder, |
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