| 吉富罗非鱼GH 基因的分离及其SNPs与增重的相关性 |
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| 引用本文： | 唐永凯. 吉富罗非鱼GH 基因的分离及其SNPs与增重的相关性[J]. 湖南农业大学学报(自然科学版), 2012, 38(4): 422-425 |
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| 作者姓名： | 唐永凯 |
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| 作者单位： | 1. 农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081
2. 南京农业大学无锡渔业学院,江苏无锡214081 |
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| 基金项目： | 农业部公益性行业科研专项(200903046–02);中央级公益性科研院所基本科研业务费专项(2011JBFC03) |
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| 摘 要： | 采用同源克隆法从吉富罗非鱼血液DNA中分离出GH基因。GH含有5个内含子,阅读框长615 bp,编码204个氨基酸。通过随机测序法,在GH的外显子6处找到1个SNPs位点(A/G)。通过PCR–RFLP检测吉富罗非鱼群体内GH的SNPs,同时将其增重性状与SNPs进行相关分析,结果显示,在雌鱼中,GG型和GA型个体增重高于AA型个体,但差异不显著;在雄鱼中,AA型个体增重显著高于GG型个体(P<0.05)。
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| 关 键 词： | 吉富罗非鱼 生长激素 多态位点 增重 |
| 收稿时间： | 2012-05-18 |
| 修稿时间： | 2012-05-18 |
Cloning of growth hormone (GH) gene of genetically improved farmed tilapia(GIFT) and association of the single nucleotide polymorphisms(SNPs) of GH gene with the weight gain |
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| TANG YONGKAI. Cloning of growth hormone (GH) gene of genetically improved farmed tilapia(GIFT) and association of the single nucleotide polymorphisms(SNPs) of GH gene with the weight gain[J]. Journal of Hunan Agricultural University, 2012, 38(4): 422-425 |
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| Authors: | TANG YONGKAI |
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| Affiliation: | 1.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Wuxi,Jiangsu 214081,China;2.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi,Jiangsu 214081,China) |
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| Abstract: | GH gene of GIFT(genetically improved farmed tilapia) was isolated from blood DNA by homology-based cloning.GH gene included five introns and an open reading frame of 615 bp which encoded 204 amino acid residues.A site in exon 6 of GH gene showed SNPs(A/G) through random sequencing.PCR–RFLP method was used to detect SNPs in GIFT population and the correlation between weight gain and SNPs was analyzed.The results showed that the weight gain of female individuals with GG and GA genotypes was higher than that of which with AA genotype,but there was no significant difference.The weight gain of male individuals with AA genotype was significantly higher than that of which with the GG genotype(P<0.05). |
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| Keywords: | tilapia growth hormone SNPs weight gain |
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