NNK和B[a]P在卷烟烟气复杂基质中的联合细胞毒性

以苯并a]芘(Ba]P)、甲基亚硝胺吡啶基丁酮(NNK)为目标化合物,设置卷烟烟气冷凝物(CSC)及NNK的质量浓度为0(CK)、0.02、0.04、0.06、0.08、0.10、0.12、0.14、0.16 mg/mL,Ba]P的质量浓度为0(CK)、0.000 8、0.0016、0.002 4、0.003 2、0.004、0.004 8、0.005 6、0.0064mg/mL,研究NNK,Ba]P和CSC对人体支气管上皮细胞(BEAS-2B)的毒性作用.结果表明:CSC的IC50为0.133 mg/mL,NNK为0.110 mg/mL,aa]P为0.004 6 mg/mL;低剂量Ba]P、NNK分别及联合与CSC作用细胞毒性均大于CSC单独作用,二者分别与CSC作用均表现为协同作用；低剂量CSC、NNK分别及联合与Ba]P作用细胞毒性均大于Ba]P单独作用,二者分别与Ba]P联合作用均表现为协同作用；低剂量CSC、Ba]P分别及联合与NNK作用的细胞毒性与NNK单独作用大小不一,二者分别与NNK作用均表现为拮抗作用；3种毒性物质联合作用均表现为拮抗作用.

Synergic cytotoxicity between NNK and B[a]P in the complicated cigarette smoke

Ba]P and NNK were used as the target compounds.CSC and NNK in the dose of 0(CK),0.02,0.04,0.06,0.08,0.10,0.12,0.14,0.16 mg/mL respectively and Ba]P in the dose of 0(CK),0.000 8,0.001 6,0.002 4,0.003 2,0.004,0.004 8,0.005 6,0.006 4 mg/mL were tested by MTT to investigate their cytotoxicity on BEAS 2B cells.The result showed the IC50 of cigarette smoke condensates(CSC),NNK and Ba]P were 0.133,0.110,0.004 6 mg/mL respectively.CSC combined with low dose of Ba]P and NNK respectively or together showed larger cytotoxicity than CSC alone,and CSC with Ba]P and NNK respectively displayed synergism.Low dose of CSC and NNK respectively or together combined with Ba]P showed cytotoxicity greater than Ba]P alone,and CSC and NNK with Ba]P respectively displayed synergism.Low dose of CSC and Ba]P respectively or together combined with NNK showed antagonism.The unification of Ba]P,CSC and NNK showed antagonism.