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| 星点设计–效应面法优化葡萄枝蔓中白藜芦醇的提取工艺 | |
| 引用本文： | 杨星星,熊兴耀. 星点设计–效应面法优化葡萄枝蔓中白藜芦醇的提取工艺[J]. 湖南农业大学学报(自然科学版), 2012, 38(3): 282-286 |
| 作者姓名： | 杨星星 熊兴耀 |
| 作者单位： | 1. 湖南农业大 学园艺园林学院,湖南 长沙,410128 2. 湖南农业大 学园艺园林学院,湖南 长沙 410128;作物种质创新与资源利用国家重点实验室,湖南 长沙410128;湖南省中药提取工程研究中心,湖南 长沙 410331 3. 湖南农业大 学园艺园林学院,湖南 长沙 410128;作物种质创新与资源利用国家重点实验室,湖南 长沙410128 |
| 摘 要： | 用乙醇回流法从葡萄枝蔓中提取白藜芦醇,以乙醇体积分数(x1)、回流时间(x2)和溶媒比(x3)为自变量,以白藜芦醇得率(y)为因变量,得二次回归方程y=–6.186 39+0.181 78x1–0.012 371x2+0.122 93x3+0.000 273x1x2–0.000 545x1x3–0.000 164x2x3–0.001 178x12–0.000 042x22–0.002 998x32(r=0.936 1,P<0.01)。用星点设计–效应面法优化提取工艺条件,优化后的较佳提取工艺条件为乙醇体积分数90.2%,提取时间129.5 min,溶媒比8.8,共提取2次。 |
| 关 键 词： | 葡萄枝蔓 白藜芦醇 提取工艺 星点设计 效应面优化法 |
| 收稿时间： | 2012-02-20 |
| 修稿时间： | 2012-04-15 |
Optimization of extraction process of resveratrol from grapevine by central composite design and response surface method |
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| yangxingxing and xiongxingyao. Optimization of extraction process of resveratrol from grapevine by central composite design and response surface method[J]. Journal of Hunan Agricultural University, 2012, 38(3): 282-286 | |
| Authors: | yangxingxing and xiongxingyao |
| Affiliation: | 1,2 (1.College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;2.Pre-State Key Laboratory for Germplasm Innovation and Resource Utilization,Changsha 410128,China;3.Herb Extract Engineering Research Center of Hunan Province,Changsha 410331,China) |
| Abstract: | Resveratrol was extracted from grapevine by ethanol reflux extraction,and the quadratic regression equation y=–6.186 39+0.181 78x1–0.012 371x2+0.122 93x3+0.000 273x1x2–0.000 545x1x3–0.000 164x2x3–0.001 178 x12 –0.000 042x22 – 0.002 998x32(r= 0.936 1,P<0.01) was obtained by selecting the ethanol concentration(x1),time for refluxing extraction(x2) and ratio of solvent to raw material(x3) as independent variables and the extraction rate of resveratrol as dependent variables.Then the extraction process was optimized using central composite design and response surface method.The optimal conditions of extraction process were as follows: extracted 2 times,129.5 min for each extraction,with 90.2% ethanol and solvent to raw material ratio of 8.8∶1. |
| Keywords: | grapevine resveratrol extraction process central composite design response surface method |
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