| 野鸟源H5N8禽流感病毒遗传变异分析与致病性评估 |
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| 引用本文： | 张醒海,李元果,王铁成,初冬,孙贺廷,孙伟洋,冯娜,谢英,何健,秦思源,彭鹏,赵永坤,杨松涛,高玉伟,夏咸柱.野鸟源H5N8禽流感病毒遗传变异分析与致病性评估[J].中国兽医学报,2019(4):646-654. |
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| 作者姓名： | 张醒海 李元果 王铁成 初冬 孙贺廷 孙伟洋 冯娜 谢英 何健 秦思源 彭鹏 赵永坤 杨松涛 高玉伟 夏咸柱 |
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| 作者单位： | 1.军事科学院军事兽医研究所/吉林省人兽共患病预防与控制重点实验室;2.吉林大学动物医学学院;3.国家林业局野生动物疫源疫病监测总站;4.中国医学科学院实验动物研究所 |
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| 基金项目： | 国家"十三五"重大科技专项资助项目(2016YFC1201602;2017YFD0500105;2016YFC1201604) |
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| 摘 要： | 本试验在野鸟禽流感病毒紧急疫情检测过程中鉴定并分离到1株H5N8高致病性禽流感病毒,利用病毒全基因组测序、系统发育及关键氨基酸位点分析解析了该野鸟源H5N8禽流感病毒分离株遗传进化情况,通过体外复制动力学试验及小鼠感染试验,评价了该野鸟源H5N8禽流感病毒分离株对哺乳动物致病性。进化分析显示,该病毒株属于Clade 2.3.4.4,可以不经适应直接感染小鼠并在呼吸系统内复制,表现出有限的组织嗜性,对小鼠呈低致病性。其在体内外复制能力较低。结果表明,本试验加深了对野生鸟携带H5N8禽流感病毒的认识和理解、对野鸟源H5N8禽流感病毒生物学特性的评价,为预测野鸟源H5N8禽流感病毒遗传进化趋势及其生物安全风险评估提供借鉴和参考。
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| 关 键 词： | 野鸟 H5N8 禽流感病毒 遗传变异 致病性 |
Genetic evolution analysis and pathogenicity evaluation of wild birds-origin H5N8 avian influenza virus |
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| ZHANG Xing-hai,LI Yuan-guo,WANG Tie-cheng,CHU Dong,SUN He-ting,SUN Wci-yang,FENG Na,XIE Ying,HE Jian,QIN Si-yuan,PENG Peng,ZHAO Yong-kun,YANG Songtao,GAO Yu-wei,XIA Xian-zhu.Genetic evolution analysis and pathogenicity evaluation of wild birds-origin H5N8 avian influenza virus[J].Chinese Journal of Veterinary Science,2019(4):646-654. |
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| Authors: | ZHANG Xing-hai LI Yuan-guo WANG Tie-cheng CHU Dong SUN He-ting SUN Wci-yang FENG Na XIE Ying HE Jian QIN Si-yuan PENG Peng ZHAO Yong-kun YANG Songtao GAO Yu-wei XIA Xian-zhu |
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| Institution: | (Key Laboratory of Jilin Province for Zoonosis Prevent io ti and Control/ Military Veterinary Research Institute, Academy of Military Sciences? Changchun 130122, China;College of Veterinary Medicine ?J ilin University , Changchun 130062 ,China;Institute of Laboratory Animat Sciences , Chinese Academy of MedicaI Sciences and Peking Unio/i Medical College , Beij in^ 100021 China;State Forestry Ad ministration Wild Animal Epidem ic Surveillance Station ^Shenyang 110034 , China) |
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| Abstract: | ZHANG Xing-hai;LI Yuan-guo;WANG Tie-cheng;CHU Dong;SUN He-ting;SUN Wci-yang;FENG Na;XIE Ying;HE Jian;QIN Si-yuan;PENG Peng;ZHAO Yong-kun;YANG Songtao;GAO Yu-wei;XIA Xian-zhu(Key Laboratory of Jilin Province for Zoonosis Prevent io ti and Control/ Military Veterinary Research Institute, Academy of Military Sciences? Changchun 130122, China;College of Veterinary Medicine ?J ilin University , Changchun 130062 ,China;Institute of Laboratory Animat Sciences , Chinese Academy of MedicaI Sciences and Peking Unio/i Medical College , Beij in^ 100021 China;State Forestry Ad ministration Wild Animal Epidem ic Surveillance Station ^Shenyang 110034 , China) |
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| Keywords: | wild birds H5N8 avian influenza genetic evolution pathogenicity |
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