外源激素对一个新的棉花极端矮化突变体AS98植株生长和酶活性的影响

【目的】探索棉花极端矮化突变体AS98株高性状对不同外源激素的敏感性,进而确定该突变体属于哪种激素缺陷型。【方法】以突变体AS98和正常型品种LHF10W99为材料,用不同浓度的GA3、IAA和BR处理AS98,比较分析不同处理条件下AS98株高、节间长度和叶片数的变化;同时,分析AS98在GA3和PP333处理后,α-淀粉酶活性、POD活性、SOD活性等的变化。【结果】表明没有用激素处理的突变体AS98的平均株高、节间长度、铃重、子指,均显著低于正常基因型LHF10W99,但是其相同时期的叶片数和衣分等性状与LHF10W99差异不显著;100mg·L-1的外源GA3连续处理后,AS98的株高和节间长度明显高于未处理的AS98对照,差异达极显著水平,而且其株高达到了正常基因型对照LHF10W99水平。但不同浓度IAA和BR处理后,AS98株高与未处理的AS98对照相比,差异不显著。回归分析也表明AS98的株高和节间长度与外源GA3具有显著的正相关性,而与外源IAA和BR的相关性不显著。此外,研究还表明GA3能诱导AS98的α-淀粉酶活性提高,降低SOD和POD活性,PP333能诱导AS98的α-淀粉酶活性降低,提高SOD和POD活性。【结论】综上可见,AS98是一个株高极端矮化、叶片为正常阔叶的新型棉花矮化突变体;AS98的株高对GA3敏感,连续用GA3处理可以恢复其株高,而且GA3和PP333均能诱导AS98的α-淀粉酶活性发生敏感性反应,AS98是一个GA3敏感型矮化突变体。

Effect of Exogenous Hormone on Plant Growth and Enzyme Activity of a Novel Cotton Dwarf-Mutant AS98

Objective] The purpose of this study was to illuminate the sensibility of the exogenous hormones to the plant height characters of a new dwarf mutant AS98 and determine which kind of hormone deficiency this mutant belongs to. Method] Two cotton lines, dwarf mutant AS98 and normal type LHF10W99, were used as materials in this research. AS98 was treated with different contents of exogenous GA_3, IAA and BR. The changes of plant height, internode length and leaf number were investigated in different treatments. Meanwhile, the activities of α-amylase, POD and SOD of the mutant were analyzed after the mutant AS98 was treated with GA_3 and PP333. Result] The result indicated that the average plant height, internode length, boll weight and seed index of AS98 without hormone treatment were markedly lower than that of normal genotype LHF10W99, respectively, but their leaf number and lint percentage were not significantly different compared with that of LHF10W99. After being treated with 100 mg·L~(-1) exogenous GA_3 continuously, the plant height and internode length of AS98 showed very significant difference compared with AS98 without being treated with hormone, and reached that of the normal type of LHF10W99. But the plant height and internode length of AS98 treated with IAA and BR did not show significant difference with that of the control. The regression analysis showed that there was a significant relationship between the exogenous GA_3 and plant height, internode length of mutant AS98, hut there was no significant relationship between the plant height and IAA and BR. Moreover, the exogenous GA_3 could increase the α-amylase activity of AS98 and decrease POD and SOD activities. On the contrary, PP333 could reduce the α-amylase activity of AS98 and up-regulate POD and SOD activities. Conclusion] It is concluded that AS98 is a new super-dwarf mutant with normal broadleaf. The plant height of AS98 is sensitive to the exogenous GA_3, and can be resumed to normal height with exogenous GA_3 continuous treatment. The α-amylase activity of AS98 is sensitive to exogenous GA_3 and PP333. AS98 is a GA_3-deficiency dwarf mutant.