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| 日本圆叶海棠组培苗的快速繁殖 | |
| 引用本文： | 欧晓峰,张建新,郭 倩,张笃峰.日本圆叶海棠组培苗的快速繁殖[J].西北农业学报,2010,19(2):189-193. |
| 作者姓名： | 欧晓峰 张建新 郭 倩 张笃峰 |
| 作者单位： | 1. 延安大学,生命科学学院,陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000 2. 陕西省果树良种苗木繁育中心,陕西铜川,727031 3. 陕西省延安中学,陕西延安,716000 |
| 基金项目： | 陕西省教育厅重点实验室项目资助 |
| 摘 要： | 以日本圆叶海棠初代培养获得的茎段为外植体,采用正交和随机区组设计培养基进行继代和生根培养.建立了日本圆叶海棠试管苗快繁体系,并优化获得其适宜继代的培养基MS + IBA 0.2 mg/L + 6-BA 0.3 mg/L + 蔗糖3% + 琼脂0.55%,适宜的生根培养基为1/2MS + IAA 0.5 mg/L +蔗糖2% + 琼脂0.55%.研究表明,MS基本培养基附加低浓度水平的6-BA与IBA的激素组合,有利于丛生芽的诱导,1/2MS基本培养基附加低浓度的IAA,有利于根的诱导,NAA与IBA不适用于生根.生根苗的移栽可采用温室自然光过渡法炼苗后,以蛭石为基质的营养钵移栽法. |
| 关 键 词： | 日本圆叶海棠(Malus prunifolia var.ringo) 茎段快繁 继代与生根培养 生根苗移栽 |
Rapid Propagation of Tissue Culture Seedling of the Japanese Malus prunifolia var . Ringo |
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| OU Xiaofeng,ZHANG Jianxin,GUO Qian,ZHANG Dufeng.Rapid Propagation of Tissue Culture Seedling of the Japanese Malus prunifolia var . Ringo[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(2):189-193. | |
| Authors: | OU Xiaofeng ZHANG Jianxin GUO Qian ZHANG Dufeng |
| Abstract: | Taken the original cultured stem sections of Japanese Malus prunifolia var.ringo as explants,the mediums for subculture and rooting culture were designed by using the orthogonal and the randomized block experiments.Then,the rapid propagation system of tube seedling of Japanese Malus prunifoliavar.ringo was well established.The results showed that the suitable medium for subculture was composed of MS + IBA 0.2 mg/L + 6-BA 0.3 mg/L + sucrose 3% + agar 0.55%,and medium for rooting culture was composed of 1/2MS... |
| Keywords: | Japanese Malus prunifolia var ringo Stem sections regeneration Subculture and rooting culture Transplanting of rooting seedlings |
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