| 马铃薯StERF3超量表达载体转基因植株矮化原因初探 |
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| 引用本文： | 刘 影,田振东,宋波涛,柳 俊,谢从华.马铃薯StERF3超量表达载体转基因植株矮化原因初探[J].华中农业大学学报,2011,30(6):663-666. |
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| 作者姓名： | 刘 影 田振东 宋波涛 柳 俊 谢从华 |
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| 作者单位： | 国家蔬菜改良中心华中分中心/华中农业大学湖北马铃薯工程技术研究中心,武汉,430070 |
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| 基金项目： | 国家自然科学基金项目(30971833); 湖北省自然科学基金杰出青年人才项目(2010CDA102) |
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| 摘 要： | 基于马铃薯StERF3超量表达载体转基因株系中出现了部分矮化植株,通过基因表达量检测、组织切片分析和转基因试管苗添加外源生长调节剂等方面对矮化原因进行了初步探讨.结果表明:矮化株中StERF3基因表达比对照降低；矮化株木质部和薄壁细胞明显变小,栅栏组织和海绵组织较致密；培养基中添加0.5 mg/mL GA3能够使矮化株...
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| 关 键 词： | 马铃薯 转基因 StERF3基因 矮化 超量表达载体 基因沉默 |
| 收稿时间： | 9/3/2010 12:00:00 AM |
Factors leading to dwarfism of transgenic potato plants transformed with StERF3 over expression vector |
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| LIU Ying,TIAN Zhen-dong,SONG Bo-tao,LIU Jun,XIE Cong-hua.Factors leading to dwarfism of transgenic potato plants transformed with StERF3 over expression vector[J].Journal of Huazhong Agricultural University,2011,30(6):663-666. |
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| Authors: | LIU Ying TIAN Zhen-dong SONG Bo-tao LIU Jun XIE Cong-hua |
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| Institution: | LIU Ying TIAN Zhen-dong SONG Bo-tao LIU Jun XIE Cong-hua National Center for Vegetable Improvement(Central China)/Potato Engineering and Technology Research Center of Hubei Province,Huazhong Agricultural University,Wuhan 430070,China |
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| Abstract: | Several dwarf plants occurred in potato lines transformed with StERF3 over expression vector and the influencing factors of the dwarfism was investigated.Gene expression test revealed that StERF3 expression level in dwarf plants was lower than that of control.StERF3 gene expression decreased in the interference transgenic lines,but no dwarf plants appeared.The results indicated that the dwarfism was not related with the expression of StERF3 gene.Medium supplemented with 0.5 mg/mL exogenous GA3 could improve... |
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| Keywords: | potato transgene StERF3 dwarf over expression vector gene silencing |
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