中华结缕草节间组织培养及无性系建立的研究

以中华结缕草的根茎节间为材料，进行了愈伤组织诱导、愈伤组织分化、不定芽生根、试管苗移栽的试验，成功地建立起中华结缕草无性系。结果表明：MS＋BA0．5＋2，4-D2．5～3．0是诱导形成具有分化能力愈伤组织的理想培养基；1／2MS＋Ag（NO3）2 0．8＋BA0．4＋NAA0．1是愈伤组织和不定芽分化的理想培养基；1／3MS＋IAA0．4是生根培养的理想培养基；移植后的试管苗根茎上的须根比野生植株增加1．5～2．0倍，其他性状与野生植株完全一致。

Tissue Culture and Construction of Clones of Zoysia sinica Hance

The leafstalk of Zoysia sinica Hance were used as explants to indue callus and differentiation, root of adventitious bud and transplant of seedling. The results showed that MS ＋ BA 0.5 ＋ 2,4-D 2.5 - 3.0 is the ideal medium for inducing callus; The opmium medium for forming callus and differentiation of adventitious bud was 1/2 MS ＋ Ag（ NO3 ）2 0.8 ＋ BA 0.4 ＋ NAA 0.1 ; 1/3 MS ＋ IAA 0.4 was suitable medium for rooting. The number of fibrous root on rhizome is 1.5 - 2.5 times than the wild, but the phenotype was the same as the wild.