| 喙荚云实种皮总黄酮的提取及黄酮类型的初步鉴定 |
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| 引用本文： | 黄明堦,陈燕丹,张福娣,魏道智.喙荚云实种皮总黄酮的提取及黄酮类型的初步鉴定[J].江西农业大学学报,2009,31(6). |
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| 作者姓名： | 黄明堦 陈燕丹 张福娣 魏道智 |
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| 作者单位： | 1. 福建农林大学,生命科学学院,福建,福州,350002
2. 福建农林大学,材料工程学院,福建,福州,350002 |
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| 基金项目： | 福建省自然科学基金项目，福建农林大学青年教师科研基金项目资助 |
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| 摘 要： | 采用L_9(3~4)正交试验表进行试验,通过极差和方差分析优化确定喙荚云实种皮总黄酮的超声波-乙醇提取条件为:乙醇浓度50%,提取温度80℃,提取时间60 min,料液比1:15,提取次数为3次,总黄酮的提取率为8.09%.通过喙荚云实种皮总黄酮提取物溶解性试验、显色反应以及产品在不同诊断试剂中的紫外-可见吸收光谱等特征判断其基本结构类型,初步确定喙荚云实种皮中应含有具有A-环邻位二羟基(6,7或7,8-)的二氢黄酮或二氢黄酮醇苷类化合物.
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| 关 键 词： | 喙荚云实 超声波 黄酮 提取 鉴定 |
Extraction of Flavonoids from the Seeds Coat of Caesalpinia minax Hance and Preliminary Identification of Flavonoids Type |
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| HUANG Ming-jie,CHEN Yan-dan,ZHANG Fu-di,WEI Dao-zhi.Extraction of Flavonoids from the Seeds Coat of Caesalpinia minax Hance and Preliminary Identification of Flavonoids Type[J].Acta Agriculturae Universitis Jiangxiensis,2009,31(6). |
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| Authors: | HUANG Ming-jie CHEN Yan-dan ZHANG Fu-di WEI Dao-zhi |
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| Abstract: | According to L_9(3~4)orthogonal experiment scheme and range and variance analysis,the con-ditions for the optimal ultrasonic assisted-ethanol extraction of the flavonoids in the seeds coat of Caesalpinia minax Hance were established as follows:extraction for three times using 50%ethanol as the extraction sol-vent with material to ethanol ratio of 1:15 and extraction for 60 min at 80℃ each time.Under the best extrac-tion conditions.the extraction rate of the flavonoids in the seeds coat of Caesalpinia minax Hance was 8.09%.Based on the solubility tests,chromogenic reaction method and the UV-vis spectra of the flavonoids dissolved in different detection reagents,the structure types of the flavonoids can be preliminary identified as a kind of flavanone with o-dihydroxy(6,7 or 7,8)in ring A or compounds of dihydro-flavonol glycosides. |
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| Keywords: | Caesalpinia minax Hance ultrasonic flavonoids extraction identification |
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