哈茨木霉菌(Trichoderma harzianum)和终极腐霉菌(Pythium ultimum)对玉米蛋白质组的影响(I)

 哈茨木霉(Trichoderma harzianum) T22菌株已普遍用于防治包括由终极腐霉(Pythium ultimum)引起的苗病或根腐病在内的各种病害。玉米自交系Mo17种子经T22处理后播种在接种腐霉或未接种的田间土壤内,5 d后取幼苗的根系或幼茎提取蛋白。结果表明:在接种腐霉菌的土壤内,未进行T22处理的5 d龄幼苗长势明显比对照差,而经T22处理的幼苗长势明显比对照好。T22和腐霉菌复合处理及T22单独处理对幼苗生长影响基本相同。本研究建立了蛋白质提取和双向电泳分离技术。通过双向电泳及相应的分析软件(PDQuest^TM 2-D softw are)可将不同处理的幼苗自交系蛋白进行分离。T22菌株处理的根系产生104种上游调控蛋白和164种下游调控蛋白,T22与腐霉菌复合处理可产生97种上游调控蛋白和150种下游调控蛋白,而用腐霉菌单一处理诱导的上游或下游蛋白的数量明显少于上述2个处理。T22或腐霉菌单一或复合处理的根系蛋白质组图谱与空白对照相比差异显著,它们与对照的蛋白质组图谱相似系数分别为0.72、0.51和0.49;T22与腐霉菌分别处理的蛋白质组图谱间也相差明显,两者的相似系数仅为0.65。进一步研究发现,T22菌体蛋白质组图谱与上述各种处理的蛋白质组图谱相似系数均很低,说明各种处理诱导后的幼苗根系蛋白质组组分主要来自植物本身,其变化主要因T22或腐霉菌的诱导所致。腐霉菌的侵染对寄主根系蛋白组图谱影响明显高于T22的作用。蛋白质组中各种蛋白质的质谱分析(M ALDI-TOF)与鉴定将另文发表。

The Change of Maize Plant Proteome Affected by Trichoderma harzianum and Pythium ultimum (Ⅰ)

Trichoderma harzianum strain T22 controls various diseases of maize and other crops,including seedling and root rots caused by Pythium ultimum.Seedlings of inbred line Mo17 were grown from T22-treated or untreated seeds in field soil or in field soil intested with the pathogen.Five days after planting,seedlings of Mo17 (5-day-old) were smaller in the presence of P.ultimum and larger in the presence of T22 relative to the control.The combination of T22 with P.ultimum (T22+P.ultimum)resulted in plants as large as T22 alone.Methods for protein extraction and 2-D gel electrophoresis were developed.Proteins in seedlings roots from the various treatments were separated on 2-D gels and analyzed using PDQuestTM 2-D software.With seedlings produced from T22-treated seeds,there were 104up-regulated proteins and 164 down-regulated proteins relative to the control,and 97 and 150 from the treatment with T22+P.ultimum,respectively,however,with P.ultimum alone the numbers were much lower than above two treatments.Comparatively,there was very lower similarity of proteome patterns of seedling roots with T22 or P.ultimum or both to control seedlings,the correlative coefficient values were 0.72,0.51 and 0.49 for the comparisons among control with T22,P.ultimum and T22+P.ultimum,respectively.Moreover,correlative coefficient of proteome patterns between T22 with P.ultimum was only 0.65,and T22 fungal proteome were also not same as any one of seedling roots with various treatments.Taken together,the components in seedling root proteome seemed to be mostly coming from Mo17 plants themselves and affected strongly by either microbes,but the effects appeared to be stronger by P.ultimum than by T22.MALDI-TOF analysis of differentially expressed proteins will publish on another paper.