牛病毒性腹泻病毒在绵羊垂直感染中的抗原分布

将新疆分离的病毒性腹泻病毒（BVDV）野毒株SHN－98和标准毒株Oregon C-24分别接种无BVDV感染的羔羊和怀孕100天左右的母羊，建立BVDV在绵羊垂直感染的动物模型，应用病毒抗原定位检测的免疫组化染色技术，探求BVDV在实验性感染绵羊经胎盘感染胚胎、羔羊过程中，病毒在感染组织、靶器官、靶细胞中的分布规律。结果表明：BVDV通过母羊的胎盘感染胚胎。BVDV在感染妊娠母羊体内主要分布于心肌、肝、肺、脾、淋巴结、胃肠粘膜、脑神经、子宫粘膜、胎盘等器官组织，其中以胃肠粘膜、脾、淋巴结、脑神经、子宫粘膜、胎盘等器官组织中分布量较多；BVDV在垂直感染胚胎体内主要分布于胸腺、淋巴结、脑、肝、肾、心肌、脾、肺、胃肠粘膜、脐带等器官组织，其中以胸腺、胃肠粘膜、脑神经等器官组织中分布量较多；BVDV在垂直感染羔羊体内主要分布于心、肾、胃肠粘膜、脾、胸腺、淋巴结、大脑、小脑、海马、视丘、视神经、眼球脉络膜等器官组织，其中以胃肠粘膜、大小脑、用、视神经、淋巴结等器官组织中分布较多，在组织分布中，BVDV对上皮细胞、神经胶质细胞、淋巴细胞有较强的亲嗜性，SHN－98和OregonC-24在垂直传播中组织器官的分布无明显差异。

Distribution of Bovine Viral Diarrhea Virus(BVDV) in Vertical Infectious Sheep

BVDV isolats from Xinjiang (SHN_98)and Standard strain OregonC_24 were inoculated pregnant sheep and lamb,and animal mode for vertical infection of BVDV was found,and BVDV antigen was tested by DAB Technology and distributed regularity for BVDV in experimental sheep and fetus and lamb was studied.The results demonstrated that BVDV distributed in muscle of heart,liver,lung,spleen,lymph node,alimentary canal,uterus mucous,cranial nerve and placenta mucous etc of pregnant sheep,and there were more BVDV in spleen,lymph node,alimentary canal,uterus mucous,cranial nerve and placenta mucous;For vertical infected embryo,BVDV were in lymph node,thymus,Brain,live,kidney,heart muscle,lung,spleen,stomach and intestine mucous and umbilical cord etc,and there were more BVDV in thymus,stomach and intestine mucous and cranial nerve;For lamb and vertical infected lamb,BVDV were in heart muscle,liver,thymus,spleen,lymph node,alimentary canal mucous,cerebrum,cerebellum,sea horse,optic nerve and cyeball vein,and there were more in alimentary canal mucous?cerebrum?cerebellum?spleen,optic nerve and lymph node,BVDV have more stronger affinity to epithelial cell?never cell and lymph cell.There was no significant difference between SHN98 and OregonC_24 in the distribution of organs or tissue.