| 矮生一品红组织培养快繁体系的建立 |
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| 引用本文： | 尹英敏,朴日子,曹后男,郑仙霞,陈蕾.矮生一品红组织培养快繁体系的建立[J].延边大学农学学报,2006,28(4):293-297. |
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| 作者姓名： | 尹英敏 朴日子 曹后男 郑仙霞 陈蕾 |
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| 作者单位： | 延边大学农学院园艺系,吉林,龙井,133400 |
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| 摘 要： | 以矮生一品红的幼叶、幼茎、茎芽为外植体，研究了影响丛芽诱导、增殖、生根壮苗的组培快繁的主要因素．结果表明：茎段、茎芽是诱导丛芽的较好试材．适合诱导胚性愈伤组织和芽分化的培养基为MS＋6-BA1．0mg／L＋NAA0．1mg／L；适合丛芽增殖成苗的培养基为MS＋6-BA1．0mg／L＋IAA0．1mg／L，继代增殖培养40d的平均增殖倍数为11；适合生根壮苗的培养基为1／2MS＋IBA0．9mg／L，开始生根天数为8d。生根率可达85％．采用河砂和营养土两步炼苗。移栽成活率可达90％以上．
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| 关 键 词： | 一品红 组织培养 快繁体系 激素 |
| 文章编号： | 1004-7999(2006)04-0293-05 |
| 收稿时间： | 2006-10-25 |
| 修稿时间： | 2006年10月25 |
Establishment of tissue culture and rapid propagation system for dwarf Euphorbia pujcher ima |
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| YIN Ying-min,PIAO Ri-zi,CAO Hou-nan,ZHENG Xian-xia,CHEN Lei.Establishment of tissue culture and rapid propagation system for dwarf Euphorbia pujcher ima[J].Journal of Agricultural Science Yanbian University,2006,28(4):293-297. |
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| Authors: | YIN Ying-min PIAO Ri-zi CAO Hou-nan ZHENG Xian-xia CHEN Lei |
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| Institution: | Horticulture Department, Agricultural College of Yanbian University, Longjing Jilin 133400, China |
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| Abstract: | The young leaves,young stem segments and stem segments with buds were used as explants to study the mainly factors which affected the cluster buds induction,multiplication and seedlings rooting and transplant of tissue culture and rapid propagation.The results showed that the stem segments and stem bud were better for inducing cluster buds.MS+6-BA 1.0 mg/L+NAA 0.1 mg/L was suitable for inducing embryoid callus and cluster buds;MS+6-BA 1.0 mg/L+IAA 0.1 mg/L was suitable for cluster buds multiplication,and the average multiplication was 11 when cultured 40 days;1/2 MS+IBA 0.9 mg/L was suitable for rooting,which started rooting eight days later,and the rooting was up to 85%.River sand and humus were used in two successive steps,and the survival rates was more than 90%. |
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| Keywords: | Euphorbia pujcher ima tissue culture rapid propagation system hormone |
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