鸭大肠杆菌自家铝胶灭活苗的制备及质量检验 |
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引用本文: | 刘玉华,方向红,王琳琳,戴丽红.鸭大肠杆菌自家铝胶灭活苗的制备及质量检验[J].安徽农业科学,2014,42(36):12929-12930. |
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作者姓名: | 刘玉华 方向红 王琳琳 戴丽红 |
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作者单位: | 江苏农牧科技职业学院,江苏泰州,225300;江苏农牧科技职业学院,江苏泰州,225300;江苏农牧科技职业学院,江苏泰州,225300;江苏农牧科技职业学院,江苏泰州,225300 |
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摘 要: | 目的]为有效控制鸭大肠杆菌病的流行提供参考依据.方法]利用分离自泰州某规模化养鸭场的大肠杆菌菌株TZY1作为菌种,制备鸭大肠杆菌铝胶灭活苗,并进行相应的质量检验.结果]通过无菌检验、安全检验和动物保护力试验发现自制疫苗无外源性微生物污染,对雏鸭安全性好.对雏鸭的攻毒试验结果表明制备疫苗的免疫保护率达100%,该疫苗的免疫剂量为0.5ml.田间试验结果表明免疫保护率达95.1%.结论]成功制备了鸭大肠杆菌铝胶灭活苗.
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关 键 词: | 鸭 大肠杆菌 铝胶 灭活苗 制备 |
Preparation and Quality Examination of Self-prepared Aluminum Hydroxide Inactivated Vaccine against Escherichia coli of Duck |
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Institution: | LIU Yu-hua, FANG Xiang-hong, WANG Lin-lin (Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu 225300) |
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Abstract: | Objective ] The research aimed to provide reference basis for controlling the prevalence of duck colibacillosis effectively. Meth-od ] TZY1 strain of Escherichia coli separated from a large-scale duck farm in Taizhou was used to prepare the aluminum hydroxide inactivatedvaccine against Escherichia coli of duck. And its quality indices were examined. Result] The results of sterilization test, safety examinationand animal protection test showed that the self-prepared vaccine wasn' t polluted by exogenous microorganisms and it had good safety to duck-lings. The results of challenge experiment on ducklings showed that the immune protection rate of the self-prepared vaccine reached 100% andits immunization dose was 0.5 ml. The results of field experiment shoed that the immune protection rate was 95.1%. Conclusion] Aluminumhydroxide inactivated vaccine against Escherichia coli of duck was successfully prepared. |
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Keywords: | Duck Escherichia coli Aluminum hydroxide Inactivated vaccine Preparation |
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