| 三种暖季型草坪草基因组DNA的提取方法 |
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| 作者姓名： | 黄春琼 刘国道 |
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| 作者单位： | 黄春琼(海南大学农学院,海南儋州,571737;中国热带农业科学院热带作物品种资源研究所,海南儋州,571737);刘国道(中国热带农业科学院热带作物品种资源研究所,海南儋州,571737)? |
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| 基金项目： | 中国热带农业科学院热带作物品种资源研究所研究生基金项目"狗牙根属植物遗传多样性研究"? |
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| 摘 要： | 采用改进CTAB法分别提取狗牙根(Cynodon dactylon)、结缕草(Zoysia spp.)、假俭草(Eremorchloa ophiuroides)3种暖季型草坪草基因组DNA.得到的DNA经核酸蛋白含量测定仪测得A260/A280值处于1.74～1.98之间、A260/A230集中处于1.74～1.99,经琼脂糖凝胶电泳检测各样品具有一条明亮主带并无拖尾现象.表明改进CTAB法提取得到的DNA完整性好,纯度高,适用于分子生物学的研究.
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| 关 键 词： | 暖季型草坪草 基因组DNA提取 |
An improved method of extracting genomic DNA from Warm season Turfgrass |
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| Abstract: | Genome DNA was extracted by the inproved method from leaves of Cynodon dactylon(L.) Pers,Zoysia spp,Eremorchloa ophiuroides.The detection performed on a nucleic acids and protein analyzer showed that A260/A280 were situated between1.74~1.98,A260/A230 were ranged from 1.74 to 1.99.The extracted products had a bright band and without tailing bands which could be observed through agar gel electrophoresis. These results showed that genomic DNA obtained by optimum methodWhich had a good integrity and high purity,and could be used in each kind of molecular biology research. |
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| Keywords: | CTAB |
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