苦马豆抗盐愈伤组织突变系的筛选

为获得苦马豆抗盐愈伤组织突变系及筛选抗盐系的适合盐浓度,采用苦马豆子叶外植体在添加1.79 mg/L 2,4-D和1.15 mg/L Kt的MS培养基上诱导的愈伤组织为材料,经不同盐浓度(100,200,280 mmol/L)条件下胁迫,并测定其在不同时间(0,3,6,9天)胁迫处理后的可溶性蛋白含量、SOD和POD活性及丙二醛与脯氨酸含量等生理指标,以确定筛选抗盐系的适合盐浓度.验证性试验显示,200 mmol/L NaCl胁迫下比100 mmol/L胁迫获得的抗性愈伤组织的数量多,而280 mmo儿胁迫下无抗性愈伤组织产生,表明获得苦马豆抗盐愈伤组织突变系的适宜盐浓度为200mmol/L NaCl.

Screening of Mutant Callus of Swainsona salsula Taubert Resistent to NaCl Stress

Explants(cotyledons) was cultured on MS medium supplemented with 1.79 mg/L 2,4-D and 1.15 mg/L Kt for 36 days.And then, Induced callus were subcultured onto the medium with the same mediun plus NaCl in different concentration for salt stress, and the physiological characters (actvities of SOD and POD,contents of soluble protein, MDA and proline) of the callus were tested after0, 3, 6, 9 days of stress. By comparing physiological indexes from different stress, 200mmol/L NaCl was defined as the most suitable salt content for screening mutants resistent to salt stress. Experiments for verification showed that the amount of callus resistent to stress obtained from 200mmol/LNaCl was much more than that from 100mmol/LNaCl stress, while no resistent callus was achieved from 280 mmol/LNaCl stress, confirming the reliability of previous prediction. The results presented here provide the base for obtaining regenerated resistent plants and investigating the resistent mechanism of salt stress of this plant.