整体显示小鼠骨骼标本的制作试验

为了从形态学上深入研究不同胎龄小鼠的骨化特点,分别采用了4种处死小鼠的方法和3种染色的方法制作了小鼠骨骼透明标本.结果发现,采用水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油.氨水漂白、甘油逐级透明的制作方法,能够获得理想的透明标本;温度可以调控标本完成的时间.本试验能够为胚胎学领域研究畸胎等骨与软骨发育病理学提供基本的试验方法.

Study on facture of transparent specimen in mice

The results of making transparence specimen of mouse skeleton were compared respectively, making mouse death in 4 different methods and dyeing them in 3 methods. The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen. Temperature controlling could reduce the time of specimen-fixed.