| 水稻条纹病毒楚雄分离物一个重组RNA序列分析 |
| |
| 作者姓名： | 程文金 吴祖建 谢联辉 |
| |
| 作者单位： | 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福州,350002? |
| |
| 基金项目： | 农业部公益性行业(农业)科研专，国家自然科学基金项目? |
| |
| 摘 要： | 为检测水稻条纹病毒(Rice stripe virus,RSV)基因组存在的重组RNA,采用RT-PCR方法扩增并克隆云南楚雄分离物(YCX07)的RNA重组片段YCX07R.克隆重组片段并测定序列.序列分析表明,YCX07R由RSVRNA2的3''端序列与RNA3的3''端序列重组而成,具有RSV基因组结构特征,采取双义编码策略,即在正、负链的5''端分别存在一个阅读框,编码两个重组蛋白YCX07R-5P、YCX07R-3P.分析推测重组更可能通过类似于脊髓灰质炎病毒(Poliovirus)的引物互补延伸机制发生.
|
| 关 键 词： | 重组RNA 双义编码 |
A recombinant RNA from Chuxiong isolate of Rice stripe virus(RSV) was sequenced |
| |
| Abstract: | A recombinant RNA of one isolate of Rice stripe virus(RSV) from Chuxiong(YCX07), Yunnan Province, China, was sequenced. The RNA was recombined from the 3'' segments of RNA2 and RNA3 of RSV. The recombinant RNA uses ambisense coding strategy and encodes two proteins, namely YCX07R-5P and YCX07R-3P. The two proteins located on the 5'' end of the plus and minus strand, respectively. This is similar to the four RNAs of RSV. It was discussed that the RNA was recombined from abortion replication or linked by two recombinant fragments through the recombinant site directly. |
| |
| Keywords: | RSV |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|
