| 农杆菌介导的植酸酶基因转化棉花的研究 |
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| 引用本文： | 郭彩菊,王省芬,张桂寅,迟吉娜,吴立强,李志坤,马峙英. 农杆菌介导的植酸酶基因转化棉花的研究[J]. 勤云标准版测试, 2009, 29(20) |
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| 作者姓名： | 郭彩菊 王省芬 张桂寅 迟吉娜 吴立强 李志坤 马峙英 |
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| 作者单位： | 河北省作物种质资源重点实验室/河北农业大学,保定,071001? |
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| 基金项目： | 转基因生物新品种培育重大专项，河北省科技支撑计划重大项目，河北省自然科学基金重点项目? |
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| 摘 要： | 为建立一种转化效率高、稳定性好的棉花农杆菌遗传转化体系,以优良的陆地棉品种晋棉14、陕724为受体材料,利用农杆菌介导的方法,将含有根特异性表达启动子的植酸酶基因(PhyA)转入供试品种的胚性愈伤中;对获得的再生植株进行PCR检测,证明PhyA已经被插入到棉花基因组中,晋棉14和陕724的转化效率分别为33%和8.3%;此外,还对影响农杆菌侵染棉花胚性愈伤转化效率的因素,如卡那霉素浓度、胚性愈伤组织生长状态、受体材料的基因型等进行了较为系统的比较研究.
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| 关 键 词： | 棉花 农杆菌介导 植酸酶基因 胚性愈伤 遗传转化 |
The Agrobacterium-Mediated PhyA Genetic Transformation for Embryogenic Calli of Cotton |
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| Abstract: | Abstract: In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli. Transformation efficiency of resistant plants about Jimian14 and Shann724 was 33% and 8.3% respectively. Effect of kanamycin concentration, the growth status of embryogenic calli and the genotype of the target material on the transformation efficicency had been assayed. The factors influencing agrobacterium-mediated cotton calli transformation were studied systematically in order to establish a highly efficient and stable genetic transformation system of cotton. |
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