| 拮抗放线菌111A202的种类鉴定及其16S rDNA序列分析 |
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| 引用本文： | 颜 霞,林雁冰,李慧芬. 拮抗放线菌111A202的种类鉴定及其16S rDNA序列分析[J]. 勤云标准版测试, 2009, 29(14) |
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| 作者姓名： | 颜 霞 林雁冰 李慧芬 |
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| 作者单位： | 西北农林科技大学生命科学学院,陕西杨凌,712100 ? |
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| 基金项目： | 陕西省自然科学基金"秦岭山区假橐吾内生放线菌的分离及其活性物质的研究"? |
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| 摘 要： | 对分离自药用植物假橐吾的一株拮抗放线菌菌株111A202进行了形态特征、培养特征、生理生化特征、细胞壁组分分析及16 S rDNA序列分析.结果表明该菌基内菌丝无横隔、不断裂,气生菌丝分枝、孢子丝波曲,孢子椭圆形,表面光滑.细胞壁化学组分Ⅰ型.以16 S rDNA序列为基础构建了包括7株相关种属菌在内的系统发育树,111A202与变铅青链霉菌(Streptomyces lividans)、微白黄链霉菌(S.albidoflavus)的16 SrDNA序列的相似性达到99.5%和99.6%.根据以上多相分类结果,放线菌111A202的形态培养特征及其细胞组分与链霉菌一致,且与微白黄链霉菌近似性很高,因此,可以将放线菌111A202定名为微白黄链霉菌111A202(S.albidoflavus 111A202).
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| 关 键 词： | 放线菌 鉴定 同源性 |
Identification of an Antagonistic Actinomycete 111A202 and Its 16S rDNA Sequence Analysisi |
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| Yan Xi,Lin Yanbing,Li Huifen. Identification of an Antagonistic Actinomycete 111A202 and Its 16S rDNA Sequence Analysisi[J]. , 2009, 29(14) |
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| Authors: | Yan Xi Lin Yanbing Li Huifen |
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| Abstract: | An antagonistic actinomycete named 111A202 was isolated from the cotton field in Xinjiang Xinjiang Uygur Autonomous Region. Its morphology, physiological and biochemical properties, cellwall pattern were characterized. The 16S rRNA gene of strain 111A202 was sequenced and analyzed for similarities to related bacterial species. Results showed that strain 111A202 is a member of the Genus Streptomyces, and the strain was named (S. albidoflavus 111A202). |
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| Keywords: | 16 S rDNA |
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