高产抗生素木霉菌株的选育及初步研究

为选育高产抗生素木霉菌株,利用在培养基中加入高浓度木霉菌代谢产物作为选择压力,通过紫外线诱变,以立枯丝核菌作为指示菌,结合平板对峙法、发酵产物平板活性测定法,筛选高产抗生素木霉菌株.经初筛、复筛共得到6株对立枯丝核菌具有较强抑制作用的突变株,进一步对突变株的发酵液进行活性测定,选育出了两株遗传稳定的高产抗生素木霉突变株TD103-UV-4和TD103-UV-13,生长速度分别是出发菌株的1.86倍和1.95倍,发酵产物抑菌活性明显提高.其中菌株TD103-UV-4发酵液对立枯丝核菌的抑制率在80%以上,并且对灰葡萄孢菌等植物病原菌有较强的抑制作用,产抗生素能力比出发菌株提高了47.1%.

Breeding and Preliminary Study of High Antibiotic-Production Trichoderma Strain

Disposed with UV., the spores of Trichoderma strains were cultivated in media adding high concentration of Trichoderma metabolites to get high antibiotic-production mutants, taking Rhizoctonia solani as indicator, and using confront culture and metabolites plate culture methods. 6 mutants with strong inhibitory action to Rhizoctonia solani were screened. According to the activity of their fermentation liquid, high producing antibiotic mutants, TD103-UV-4 and TD103-UV-13 with good genetic stability, were gained, and the growth rates were 1.86 and 1.95 times compared with the original strain respectively, and the activity of fermented product improved obviously. The inhibitory rate of metabolites produced by TD103-UV-4 against RS was more than 80%, and the antibiotic production capacity increased by 47.1%, meanwhile, its metabolites had a strong inhibition against other phytopathogenic fungi, such as Botrytis cinerea.