四种真菌基因组编码的细胞壁降解酶生物信息学分析

为了比较病原性真菌和非病原性真菌基因组编码的植物细胞壁降解酶,用BLAST(Basic Local Alignment Search tool)结合人工二次筛选的方法确定了四种真菌基因组编码的多种植物细胞壁降解酶,并用SignalP来判断细胞壁降解酶能否被分泌到细胞外.结果表明病原性真菌和非病原性真菌基因组编码的植物细胞壁降解酶在种类和数量上存在着明显的差异,其中E3(endoglucanase Ⅱ)仅由病原性真菌基因组编码,而且能够被分泌到细胞外,可能与病原性真菌对植物的致病性有着密切的关系.

Bioinformatic analysis of plant cell wall degrading enzymes in the genomes of four different fugi

This paper presents a comprehensive study on identifying the number of genes encoding different classes of plant cell wall degrading enzymes in the genomes of pathogenic and nonpathogenic fungi. Plant pathogenic fungi secrete extracellular enzymes that are able to degrade the plant cell walls of their host plants. These plant cell wall degrading enzymes are so necessary for penetration of the cell wall barriers, as well as for generation of simple molecules that can be assimilated for growth. Initially, BLAST was exerted to compare pathogenic (M.grisea, F.graminearum) and non?pathogenic fungi (N.crassa, A.nidulans) and the top hits of them were acquired. Then SignalP were performed to verify whether these PCWDEs would be secreted by the fungi.Consequently, we conclude that some significant biological differences of PCWDEs can be discovered between pathogenic and nonpathogenic fungi.