| 长白猪甘露聚糖结合凝集素-C基因的克隆与序列分析 |
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| 作者姓名： | 刘玉芬 刘洪雨 姚 欣 高 洋 |
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| 作者单位： | 刘玉芬,姚欣,高洋(哈尔滨师范大学生命科学与技术学院,哈尔滨,150025);刘洪雨(黑龙江省动物卫生监督所,哈尔滨,150095)? |
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| 基金项目： | 哈尔滨师范大学青年学术骨干资助计划项目"猪甘露聚糖结合凝集素基因多态性与TGEV感染相关性的研究"? |
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| 摘 要： | 探讨猪甘露聚糖结合凝集素-C基因的分子特征,从分子免疫学角度为猪的抗病育种研究提供理论依据.从长白猪肝脏组织中提取总RNA,经RT-PCR技术扩增pMBL-C基因,与pMD18-T载体连接,获得pMBL-C基因.序列测定结果显示该基因编码区为723bp,编码241个氨基酸残基.与已发表的猪MBL-C具有99%同源性;进化树分析显示与哺乳动物的MBL-C基因,尤其是牛的亲缘关系最近,而与MBL-A基因相差较大,与禽类的最远.该研究为进一步研究猪MBL分子的遗传特征提供了依据.
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| 关 键 词： | 长白猪 甘露聚糖结合凝集素C 基因克隆 序列分析 |
Cloning and sequence analysis of Porcine Mannan-binding Lectin C Gene from Landrace |
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| Abstract: | Abstract: Total RNA was extracted from liver cells of landrace, pMBL-C gene was amplified by RT-PCR. It was cloned to pMD18-T vector, then the mannan-binding lectin A gene of landrace was obtained.The sequence result indicated that the full-length of pMBL-C gene was 723bp, encoded 241 amino acid. There was 99% homology with pMBL-C published in GenBank at nucleotide and amino acid levels. There were the nearest homology with MBL-C from mammals at amino acid phylogenetic tree, particularly cattle, but the similarity had big difference with MBL-A, even for chicken. The study provides papers for the research of MBLs of pigs. |
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