SRAP标记技术及其在蔬菜作物遗传多样性分析中的应用

SRAP标记技术是基于内含子、启动子3’端含AATT核心和开放阅读框的编码区富含GC的序列规律进行随机扩增而获得DNA多态性的。由于不同的生物个体其基因组的内含子、启动子与外显子的间隔长度不同，因而扩增出的DNA指纹图谱也就产生了多态性。SRAP标记是近年来发展起来的一种DNA多态性分子标记, 以其操作简便快速、成本低、可信度高、易于测序等特点倍受关注。在短短的几年时间内，此标记已在马铃薯、水稻、苹果、柑橘类果树、樱桃、梅子、油菜、大蒜、芹菜和棉花等植物中实验应用，显示出良好的应用效果。本文综述了SRAP标记技术原理特点和在蔬菜遗传多样性研究领域初步应用情况。

SRAP Marker Technique and Its Application in Genetic Diversity Analyses of Vegetable Crops

SRAP (sequence-related amplified polymorphisms) is based on random amplification from sequences in coding sequences of ORFs which generally contain many GC bases and in promoter and intron sequences with an AATT core in 3’ region. The exon-intron markers often results with relatively high DNA polymorphism because the interval sequence length between intron and extron varies in genomes of different individuals. The SRAP marker system is a relatively recently developed DNA polymorphism technique with several advantages over other systems: simplicity, reasonable throughput rate, low cost, high reliability, easy isolation of bands for sequencing, which received concerns widely. In the last few years, it has been used in genetic diversity analysis in many plants like Brassica vegetables, potato, garlic, celery, rice, cherry, and apple. In this paper, the technical mechanism of SRAP, process of experiment protocol and its application in genetic diversity analysis of vegetable plants had been widely reviewed.