瓜尔豆品种遗传关系分析

瓜尔 [Cyamopsis tetragonoloba(L.)Taub.]为一年生抗旱耐热豆类植物,是Cyamopsis属中经济性最为重要的一个种.有关商业品种遗传关系的信息对于瓜尔豆育种至关重要.依据6个瓜尔豆,1个C. Senegalensis种和1个C.serrata种的引种系的RAPD和植物学性状数据采用UPGMA方法进行了聚类分析.供试的3个种共扩增出29条带,其中26条具有多态性.每个引物扩增出的多态性片段为2至6条,平均3.3条.聚类分析将这3个种分为2组:第一组包括C.serrata和C.senegalenzis 2个野生种,第二组包括Cyamopsis tetragonoloba种的6个品种.在第二组中,''Kinman''与其他5个品种的关系较远.用这8个材料形态性状非相似系数进行的聚类分析获得了类似的结果,但用形态性状比之RAPD检测到组内更多的变异.

Genetic relationship of guar commercial cultivars

Guar [Cyamopsis tetragonoloba (L.) Taub.] is a drought and heat tolerant annual legume and is the most economically important species in the Cyamopsis genus. Information about the genetic relationships among the commercial cultivars is important for future guar breeding programs. Eight accessions including 6 guar commercial cultivars and 1 plant introduction (PI) each of C. senegalensis and C. serrata, respectively, were studied with UPGMA (unweighted pair group method using arithmetic averages) clustering analysis using random amplified polymorphic DNA (RAPD) and morphological data. The RAPD analysis identified a total of 29 bands among the three species, with 26 bands being polymorphic. The number of polymorphic bands amplified per primer ranged from 2 to 6, with a mean of 3.3. Cluster analysis grouped the 3 species into 2 distinct groups. Group 1 consisted of accessions from C. serrata and C. senegalensis, and group 2 consisted of the 6 guar cultivars. Within group 2, Kinman is more distant from the other 5 guar cultivars. Similar results were obtained using ten morphological characters while more variability was detected within group 2 compared with that of RAPD data. Due to the low variability and narrow genetic background of commercial cultivars of guar, more guar germplasm should be introduced in order to breed out new guar cultivars.