Proton translocating ATPase mediated fungicidal activity of eugenol and thymol |
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Authors: | Aijaz Ahmad Amber Khan Snowber Yousuf Luqman A Khan Nikhat Manzoor |
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Institution: | a Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India. |
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Abstract: | Eugenol (1) and thymol (2) exhibit excellent fungicidal activity against pathogenic yeasts, including isolates resistant to azoles. The rapid irreversible action of compound-1 and compound 2 on fungal cells suggested a membrane-located target for their action. We investigated their effect on H+-ATPase mediated H+-pumping by various Candida species. Both compounds inhibit H+-ATPase activity at their respective MIC values — 500 and 100 μg/ml. Glucose stimulated H+-extrusion was also inhibited significantly by compound 1 and compound 2. Inhibition of H+-ATPase leads to intracellular acidification and cell death. Inhibition of cell growth and H+-efflux by test compounds suggests that their antifungal properties are related to their inhibitory effects on H+-ATPase. |
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Keywords: | Eugenol Thymol Plasma membrane H+-ATPase Intracellular pH Candida |
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