| 核桃早实性相关性状的SCAR标记 |
| |
| 引用本文： | 牛建新,吕建强,王林,叶春秀,张虎平,张录霞. 核桃早实性相关性状的SCAR标记[J]. 果树学报, 2008, 25(5): 732-735 |
| |
| 作者姓名： | 牛建新 吕建强 王林 叶春秀 张虎平 张录霞 |
| |
| 作者单位： | 石河子大学农学院园艺系,新疆石河子832003 |
| |
| 摘 要： | 用RAPD技术对新疆核桃早实特性的分子标记进行了研究。用180个10-mer随机引物分别扩增早实和晚实近等基因池DNA筛选出5个多态性引物,结果只有引物OPG15(5′-ACTGGGACTC-3′)扩增得到1条约710bp的早实核桃特异片段。对该片段进行克隆和序列分析,并根据序列分析结果将该RAPD标记转化为重复性和特异性更好的SCAR标记。研究设计出了1对早实核桃特异SCAR引物P1(5'-ACTGGGACTCCAATTGTATC-3')和P2(5'-ACTGGGACTCTCAACTAT-3'),用这对特异引物对14份材料进行PCR扩增,结果所有晚实核桃材料无任何扩增,但早实材料均扩增出了预期大小759bp的特异带。
|
| 关 键 词： | 核桃 早实 晚实 RAPD SCAR |
SCAR marker linked to early-bearing genes in walnut |
| |
| NIU Jian-xin,LV Jian-qiang,WANG Lin,YE Chun-xiu,ZHANG Hu-ping,ZHANG Lu-xia. SCAR marker linked to early-bearing genes in walnut[J]. Journal of Fruit Science, 2008, 25(5): 732-735 |
| |
| Authors: | NIU Jian-xin LV Jian-qiang WANG Lin YE Chun-xiu ZHANG Hu-ping ZHANG Lu-xia |
| |
| Affiliation: | NIU Jian-xin,LV Jian-qiang,WANG Lin,YE Chun-xiu,ZHANG Hu-ping,ZHANG Lu-xia(Department of Horticulture,Agricultural College,Shihezi University,Shihezi,Xinjiang 832003 China) |
| |
| Abstract: | RAPD was adopted to explore the molecular markers of early-bearing genes of walnut grown in Xinjiang.180 10-mer random primers were used to amplify the DNA in early-bearing and late-bearing near isogenic pools.Five polymorphic primers were screened out of which only OPG15(5'-ACT GGGACT-3')was capable of repeatedly amplifying a specific band of about 710 bp(OPG15710)from the early-bearing near isogenic pool and its individuals but not from the late bearing near isogenic pool.The specific band was cloned and ... |
| |
| Keywords: | RAPD SCAR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
