Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats |
| |
Authors: | Marinaro Mariarosaria Tempesta Maria Tarsitano Elvira Camero Michele Losurdo Michele Buonavoglia Canio Rezza Giovanni |
| |
Institution: | Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome 00161, Italy. mariarosaria.marinaro@iss.it |
| |
Abstract: | The analysis of cytokines secreted by antigen-specific lymphocytes is hampered in goats by the paucity of species-specific reagents yet it is crucial to study immune responses to infections. To overcome this limit, two commercial kits designed to measure soluble bovine IL-4 (by ELISA) and frequencies of bovine IFN-gamma secreting cells (by ELISPOT) were tested for cross-reactivity in goats. In addition, an ELISA specific to bovine/ovine IL-4 and employing two monoclonal antibodies, clones CC313 and CC314, was tested as well. Concanavalin A-stimulated caprine peripheral blood mononuclear cells (PBMCs) cultures were studied and they exhibited high levels of soluble IL-4 and high frequencies of IFN-gamma secreting cells. In addition, the two IL-4 ELISAs detected similar amounts of cytokine. To start defining the cytokine response triggered by caprine herpesvirus 1 (CpHV-1) infection, PBMC cultures were setup from goats naturally or experimentally infected with CpHV-1. High frequencies of IFN-gamma producing cells and low, when detectable, levels of soluble IL-4 were observed in CpHV-1-specific PBMC cultures from both groups of infected goats. Thus, the availability of cross-reactive research tools can expand cytokine studies in goats and can implement the research on immunity to other caprine infections. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|