北京鸭肌肉生长抑制素基因多态与屠体性状关系分析

肌肉生长抑制素（Myostatin，MSTN）基因在发育和成熟的骨骼肌中特异表达，对肌肉具有负调控作用。应用聚合酶链式反应单链构象多态性（PCR-SSCP）的方法，对6周龄北京鸭2个群体（Z2系和Z4系）共200个个体Myostatin基因5’调控区进行SNP分析，并对所发现的单核苷酸多态性与胴体重、胸肌重、腿肌重、全净膛重、皮脂重、腹脂重、骨架重、胸肌率、腿肌率、皮脂率、腹脂率进行了关联分析。结果发现，A1/A2位点基因型（AA和AB型）对6周龄Z2系和Z4系北京鸭的腹脂重（P＜0.05）和胸肌重（P＜0.10）有显著影响，因此可以将Myostatin基因作为影响北京鸭Z2系和Z4系腹脂重和胸肌重的候选基因。

Analysis on Gene Polymorphism of Myostatin and Carcass Traits of Peking Duck

Myostatin gene is expressed specifically in developing and mature skeletal muscle,and the expression products of myostatin gene inhibits muscle growth and differentiation.The polymorphism of the Peking duck myostatin gene was researched by PCR-SSCP.The SSCP was found in the myostatin gene promoter region.It showed two genotypes(AA,AB)in Z2 line and Z4 line of Peking ducks.The relationship between polymorphism in promoter and productive performance was analyzed.Variance analysis showed that AA genotype birds had significant higher weight of abdominal fat(P<0.05)and breast meat(P<0.10) than AB genotype in different lines.Therefore,Myostatin gene could be taken as the candidate gene.